Product: C1orf31 Antibody
Catalog: DF12867
Description: Rabbit polyclonal antibody to C1orf31
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.: 14 kDa; 14kD(Calculated).
Uniprot: Q5JTJ3
RRID: AB_2845828

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
C1orf31 Antibody detects endogenous levels of total C1orf31.
RRID:
AB_2845828
Cite Format: Affinity Biosciences Cat# DF12867, RRID:AB_2845828.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C1orf31; CA031_HUMAN; Chromosome 1 open reading frame 31; COA6; Cytochrome c oxidase assembly factor 6 homolog; Hypothetical protein LOC388753; RP5-827C21.3; Uncharacterized protein C1orf31;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGPGGPLLSPSRGFLLCKTGWHSNRLLGDCGPHTPVSTALSFIAVGMAAPSMKERQVCWGARDEYWKCLDENLEDASQCKKLRSSFESSCPQQWIKYFDKRRDYLKFKEKFEAGQFEPSETTAKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
82
Zebrafish
44
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5JTJ3 As Substrate

Site PTM Type Enzyme
S84 Phosphorylation
S85 Phosphorylation
K96 Ubiquitination
K110 Ubiquitination

Research Backgrounds

Function:

Involved in the maturation of the mitochondrial respiratory chain complex IV subunit MT-CO2/COX2. Thereby, may regulate early steps of complex IV assembly. Mitochondrial respiratory chain complex IV or cytochrome c oxidase is the component of the respiratory chain that catalyzes the transfer of electrons from intermembrane space cytochrome c to molecular oxygen in the matrix and as a consequence contributes to the proton gradient involved in mitochondrial ATP synthesis. May also be required for efficient formation of respiratory supercomplexes comprised of complexes III and IV.

Subcellular Location:

Mitochondrion intermembrane space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with COA1. Found in a complex with TMEM177, COX20, MT-CO2/COX2, COX18, SCO1 and SCO2. Interacts with MT-CO2/COX2 and SCO2. Interacts with SCO1. Interacts with COX20 in a MT-CO2/COX2- and COX18-dependent manner. Interacts with COX16.

Family&Domains:

Belongs to the cytochrome c oxidase subunit 6B family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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