C4BPA Antibody | Affinity Biosciences

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C4BPA Antibody - Western blot analysis of extracts from HepG2 cells, using C4BPA Antibody.

C4BPA Antibody - DF12869 at 1/100 staining human colorectal cancer by IHC-P.

Product:	C4BPA Antibody
Catalog:	DF12869
Description:	Rabbit polyclonal antibody to C4BPA
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	70 kDa; 67kD(Calculated).
Uniprot:	P04003
RRID:	AB_2845830

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

C4BPA Antibody detects endogenous levels of total C4BPA.

RRID:

AB_2845830
Cite Format: Affinity Biosciences Cat# DF12869, RRID:AB_2845830.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C4b-binding protein alpha chain; C4bp; C4bpa; C4BPA_HUMAN; Complement component 4 binding protein alpha; Complement component 4 binding protein; Proline-rich protein; PrP; RP11-164O23.4;

Immunogens

Immunogen:

Uniprot:

P04003(C4BPA_HUMAN) >>Visit HPA database.

Gene(ID):

C4BPA(722)

Expression:

P04003 C4BPA_HUMAN:

Chylomicrons in the plasma.

Sequence:

P04003 C4BPA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHPPKTPSGALHRKRKMAAWPFSRLWKVSDPILFQMTLIAALLPAVLGNCGPPPTLSFAAPMDITLTETRFKTGTTLKYTCLPGYVRSHSTQTLTCNSDGEWVYNTFCIYKRCRHPGELRNGQVEIKTDLSFGSQIEFSCSEGFFLIGSTTSRCEVQDRGVGWSHPLPQCEIVKCKPPPDIRNGRHSGEENFYAYGFSVTYSCDPRFSLLGHASISCTVENETIGVWRPSPPTCEKITCRKPDVSHGEMVSGFGPIYNYKDTIVFKCQKGFVLRGSSVIHCDADSKWNPSPPACEPNSCINLPDIPHASWETYPRPTKEDVYVVGTVLRYRCHPGYKPTTDEPTTVICQKNLRWTPYQGCEALCCPEPKLNNGEITQHRKSRPANHCVYFYGDEISFSCHETSRFSAICQGDGTWSPRTPSCGDICNFPPKIAHGHYKQSSSYSFFKEEIIYECDKGYILVGQAKLSCSYSHWSAPAPQCKALCRKPELVNGRLSVDKDQYVEPENVTIQCDSGYGVVGPQSITCSGNRTWYPEVPKCEWETPEGCEQVLTGKRLMQCLPNPEDVKMALEVYKLSLEIEQLELQRDSARQSTLDKEL

PTMs - P04003 As Substrate

P04003

Site	PTM Type	Source
S187	Phosphorylation	Uniprot
N221	N-Glycosylation	Uniprot
S469	Phosphorylation	Uniprot
Y470	Phosphorylation	Uniprot
S471	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
S495	Phosphorylation	Uniprot
N506	N-Glycosylation	Uniprot
N528	N-Glycosylation	Uniprot

Research Backgrounds

P04003_C4BPA_HUMAN

Function:

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Subcellular Location:

Secreted.

Tissue Specificity:

Chylomicrons in the plasma.

Subunit Structure:

Disulfide-linked complex of alpha and beta chains of 3 possible sorts: a 570 kDa complex of 7 alpha chains and 1 beta chain, a 530 kDa homoheptamer of alpha chains or a 500 kDa complex of 6 alpha chains and 1 beta chain. The central body of the alpha chain homopolymer supports tentacles, each with the binding site for C4b at the end.

(Microbial infection) Interacts with Staphylococcus aureus protein SdrE; this interaction inhibits complement-mediated bacterial opsonization.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). The role of S-palmitoylation of C4BPA in regulating murine sperm motility and complement resistance. International journal of biological macromolecules, 2024 (PubMed: 39370067) [IF=7.7]

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C4BPA Antibody - #DF12869