Product: C5orf4 Antibody
Catalog: DF12870
Description: Rabbit polyclonal antibody to C5orf4
Application: WB IHC
Reactivity: Human
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 kDa; 39kD(Calculated).
Uniprot: Q96IV6
RRID: AB_2845831

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(83%), Horse(83%), Sheep(100%), Rabbit(91%), Dog(83%)
Clonality:
Polyclonal
Specificity:
C5orf4 Antibody detects endogenous levels of total C5orf4.
RRID:
AB_2845831
Cite Format: Affinity Biosciences Cat# DF12870, RRID:AB_2845831.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q96IV6 FXDC2_HUMAN:

Down-regulated in primary acute myeloid leukemia (AML) patients.

Sequence:
MKGEAGHMLHNEKSKQEGHIWGSMRRTAFILGSGLLSFVAFWNSVTWHLQRFWGASGYFWQAQWERLLTTFEGKEWILFFIGAIQVPCLFFWSFNGLLLVVDTTGKPNFISRYRIQVGKNEPVDPVKLRQSIRTVLFNQCMISFPMVVFLYPFLKWWRDPCRRELPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYHLPFLPSPEFHDYHHLKFNQCYGVLGVLDHLHGTDTMFKQTKAYERHVLLLGFTPLSESIPDSPKRME

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Rabbit
91
Pig
83
Horse
83
Dog
83
Xenopus
75
Chicken
75
Zebrafish
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IV6 As Substrate

Site PTM Type Enzyme
K13 Acetylation
K15 Acetylation
S23 Phosphorylation
S328 Phosphorylation

Research Backgrounds

Function:

Promotes megakaryocyte differentiation by enhancing ERK phosphorylation and up-regulating RUNX1 expression.

Subcellular Location:

Cytoplasm. Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Down-regulated in primary acute myeloid leukemia (AML) patients.

Family&Domains:

Belongs to the sterol desaturase family.

Restrictive clause

 

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.