DDX10 Antibody - #DF12934

(1)  
Product: DDX10 Antibody
Catalog: DF12934
Description: Rabbit polyclonal antibody to DDX10
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 101 kDa; 101kD(Calculated).
Uniprot: Q13206
RRID: AB_2845895

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Sheep(91%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
DDX10 Antibody detects endogenous levels of total DDX10.
RRID:
AB_2845895
Cite Format: Affinity Biosciences Cat# DF12934, RRID:AB_2845895.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q13206(DDX10_HUMAN) >>Visit HPA database.

Gene(ID):
DDX10(1662)
Expression:
Q13206 DDX10_HUMAN:

High in testis but widely expressed.

Sequence:
MGKTANSPGSGARPDPVRSFNRWKKKHSHRQNKKKQLRKQLKKPEWQVERESISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLLQKKVPVKEIKINPEKLIDVQKKLESILAQDQDLKERAQRCFVSYVRSVYLMKDKEVFDVSKLPIPEYALSLGLAVAPRVRFLQKMQKQPTKELVRSQADKVIEPRAPSLTNDEVEEFRAYFNEKMSILQKGGKRLEGTEHRQDNDTGNEEQEEEEDDEEEMEEKLAKAKGSQAPSLPNTSEAQKIKEVPTQFLDRDEEEEDADFLKVKRHNVFGLDLKDEKTLQKKEPSKSSIKKKMTKVAEAKKVMKRNFKVNKKITFTDEGELVQQWPQMQKSAIKDAEEDDDTGGINLHKAKERLQEEDKFDKEEYRKKIKAKHREKRLKEREARREANKRQAKAKDEEEAFLDWSDDDDDDDDGFDPSTLPDPDKYRSSEDSDSEDMENKISDTKKKQGMKKRSNSEVEDVGPTSHNRKKARWDTLEPLDTGLSLAEDEELVLHLLRSQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Sheep
91
Dog
91
Bovine
82
Chicken
78
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13206 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
S7 Phosphorylation
S10 Phosphorylation
S52 Phosphorylation
K62 Ubiquitination
K78 Ubiquitination
K82 Ubiquitination
Y89 Phosphorylation
T93 Phosphorylation
K97 Ubiquitination
K107 Ubiquitination
K114 Ubiquitination
K179 Ubiquitination
T197 Phosphorylation
T208 Phosphorylation
T249 Phosphorylation
T255 Phosphorylation
K261 Ubiquitination
K269 Ubiquitination
Y273 Phosphorylation
K279 Ubiquitination
Y292 Phosphorylation
S302 Phosphorylation
Y305 Phosphorylation
Y358 Phosphorylation
K410 Ubiquitination
K446 Ubiquitination
K453 Acetylation
K453 Ubiquitination
K465 Ubiquitination
K515 Ubiquitination
K522 Ubiquitination
S539 Phosphorylation
T569 Phosphorylation
T577 Phosphorylation
S606 Phosphorylation
T610 Phosphorylation
S611 Phosphorylation
R626 Methylation
K657 Ubiquitination
S662 Phosphorylation
S663 Phosphorylation
K666 Acetylation
K670 Acetylation
T717 Phosphorylation
S780 Phosphorylation
Y801 Phosphorylation
S803 Phosphorylation
S804 Phosphorylation
S807 Phosphorylation
S809 Phosphorylation
S829 Phosphorylation
S831 Phosphorylation
S840 Phosphorylation
S873 Phosphorylation
S875 Phosphorylation

Research Backgrounds

Function:

Putative ATP-dependent RNA helicase.

Tissue Specificity:

High in testis but widely expressed.

Family&Domains:

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.