Product: DDX43 Antibody
Catalog: DF12936
Description: Rabbit polyclonal antibody to DDX43
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse
Mol.Wt.: 73 kDa; 73kD(Calculated).
Uniprot: Q9NXZ2
RRID: AB_2845897

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(80%), Horse(80%)
Clonality:
Polyclonal
Specificity:
DDX43 Antibody detects endogenous levels of total DDX43.
RRID:
AB_2845897
Cite Format: Affinity Biosciences Cat# DF12936, RRID:AB_2845897.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cancer/testis antigen 13; CT13; DDX43; DDX43_HUMAN; DEAD box protein 43; DEAD box protein HAGE; HAGE; Helical antigen; Probable ATP dependent RNA helicase DDX43; Probable ATP-dependent RNA helicase DDX43;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NXZ2 DDX43_HUMAN:

Expressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.

Sequence:
MSHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEAVAAGHEELPLCFALKSHFVGAVIGRGGSKIKNIQSTTNTTIQIIQEQPESLVKIFGSKAMQTKAKAVIDNFVKKLEENYNSECGIDTAFQPSVGKDGSTDNNVVAGDRPLIDWDQIREEGLKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEKRPIPNPTCTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFKAHQQKREMERKMERPQGRPKKFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Bovine
80
Pig
63
Sheep
63
Zebrafish
63
Chicken
63
Rabbit
63
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NXZ2 As Substrate

Site PTM Type Enzyme
R47 Methylation
R85 Methylation
K89 Ubiquitination
S110 Phosphorylation
S202 Phosphorylation
T203 Phosphorylation
T323 Phosphorylation
Y339 Phosphorylation
S346 Phosphorylation
T372 Phosphorylation
T544 Phosphorylation
T592 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.

Family&Domains:

Belongs to the DEAD box helicase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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