DNPEP Antibody - #DF12954
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| Product: | DNPEP Antibody |
| Catalog: | DF12954 |
| Description: | Rabbit polyclonal antibody to DNPEP |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 55 kDa; 52kD(Calculated). |
| Uniprot: | Q9ULA0 |
| RRID: | AB_2845915 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
DNPEP Antibody detects endogenous levels of total DNPEP.
RRID:
AB_2845915
Cite Format: Affinity Biosciences Cat# DF12954, RRID:AB_2845915.
Cite Format: Affinity Biosciences Cat# DF12954, RRID:AB_2845915.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Aspartyl aminopeptidase; ASPEP; DAP; DNPEP; DNPEP_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q9ULA0 DNPEP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQVAMNGKARKEAVQTAAKELLKFVNRSPSPFHAVAECRNRLLQAGFSELKETEKWNIKPESKYFMTRNSSTIIAFAVGGQYVPGNGFSLIGAHTDSPCLRVKRRSRRSQVGFQQVGVETYGGGIWSTWFDRDLTLAGRVIVKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINENFGPNTEMHLVPILATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHLGLSPKDIVEMELCLADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNAVSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAMHSIREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9ULA0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T16 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| C327 | S-Nitrosylation | Uniprot | |
| Y355 | Phosphorylation | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitous.
Subunit Structure:
Tetrahedron-shaped homododecamer built from six homodimers.
Family&Domains:
Belongs to the peptidase M18 family.
References
1). FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast cancer metastasis. PLoS biology, 2023
(PubMed: 38134227)
[IF=7.8]
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