DOC2B Antibody - #DF12955
| Product: | DOC2B Antibody |
| Catalog: | DF12955 |
| Description: | Rabbit polyclonal antibody to DOC2B |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine |
| Mol.Wt.: | 46 kDa; 46kD(Calculated). |
| Uniprot: | Q14184 |
| RRID: | AB_2845916 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12955, RRID:AB_2845916.
Fold/Unfold
Doc2 beta; Doc2-beta; Doc2b; DOC2B_HUMAN; DOC2BL; Double C2 like domain containing protein beta; Double C2 like domains beta; Double C2 like domains beta like; Double C2-like domain-containing protein beta; OTTMUSP00000006522; RP23 78H4.2;
Immunogens
Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level).
- Q14184 DOC2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTLRRRGEKATISIQEHMAIDVCPGPIRPIKQISDYFPRFPRGLPPDAGPRAAAPPDAPARPAVAGAGRRSPSDGAREDDEDVDQLFGAYGSSPGPSPGPSPARPPAKPPEDEPDADGYESDDCTALGTLDFSLLYDQENNALHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKLRTKTLRNTLNPTWNETLTYYGITDEDMIRKTLRISVCDEDKFRHNEFIGETRVPLKKLKPNHTKTFSICLEKQLPVDKTEDKSLEERGRILISLKYSSQKQGLLVGIVRCAHLAAMDANGYSDPYVKTYLRPDVDKKSKHKTAVKKKTLNPEFNEEFCYEIKHGDLAKKSLEVTVWDYDIGKSNDFIGGVVLGIHAKGERLKHWFDCLKNKDKRIERWHTLTSELPGAVLSD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14184 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S34 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot |
Research Backgrounds
Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes (By similarity).
Cytoplasm. Cytoplasmic granule. Cell membrane>Peripheral membrane protein.
Note: Translocates to the plasma membrane in a calcium-dependent manner.
Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level).
Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion. May interact with UNC13A; the interaction mediates targeting to the plasma membrane (By similarity). Interacts with cytoplasmic dynein light chain DYNLT1.
C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes (By similarity).
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