DOCK8 Antibody - #DF12957
| Product: | DOCK8 Antibody |
| Catalog: | DF12957 |
| Description: | Rabbit polyclonal antibody to DOCK8 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 230 kDa; 239kD(Calculated). |
| Uniprot: | Q8NF50 |
| RRID: | AB_2845918 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12957, RRID:AB_2845918.
Fold/Unfold
1200017A24Rik; Dedicator of cytokinesis protein 8; DOCK8; DOCK8_HUMAN; Epididymis luminal protein 205; HEL 205; MRD2; ZIR8;
Immunogens
- Q8NF50 DOCK8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATLPSAERRAFALKINRYSSAEIRKQFTLPPNLGQYHRQSISTSGFPSLQLPQFYDPVEPVDFEGLLMTHLNSLDVQLAQELGDFTDDDLDVVFTPKECRTLQPSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEICGFKKTGSRKDFHKTLPKQTFESETLECSEPAAQAGPRHLNVLCDVSGKGPVTACDFDLRSLQPDKRLENLLQQVSAEDFEKQNEEARRTNRQAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKLLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVAASSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIGDCAEPYTVIKESDGGKSKEKIEKLKLQAESFCQRLGKYRMPFAWAPISLSSFFNVSTLEREVTDVDSVVGRSSVGERRTLAQSRRLSERALSLEENGVGSNFKTSTLSVSSFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGLLRLEISTAPEIINCCLTPEMLPVKPFPENRTRPHKEILEFPTREVYVPHTVYRNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKISEMALEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLSKDQHGRNCLLASYVHYVFRLPEVQRDVPKSGAPTALLDPRSYHTYGRTSAAAVSSKLLQARVMSSSNPDLAGTHSAADEEVKNIMSSKIADRNCSRMSYYCSGSSDAPSSPAAPRPASKKHFHEELALQMVVSTGMVRETVFKYAWFFFELLVKSMAQHVHNMDKRDSFRRTRFSDRFMDDITTIVNVVTSEIAALLVKPQKENEQAEKMNISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSNLPTLISMRLEFLRILCSHEHYLNLNLFFMNADTAPTSPCPSISSQNSSSCSSFQDQKIASMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSAIHSLLSSHDLDPRCVKPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQNVALAIAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPSTQLNRILDLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMRRRAPGNDRFPGLNENLRWKKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSLNCDQSTTYLTHCFATLRALIAKFGDLLFEEEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMSLASLVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGTYFRVGFFGSKFGDLDEQEFVYKEPAITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQYRRNTVLTTMHAFPYIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLAVAINQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSFRKCETQLSQGS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NF50 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T366 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| Y559 | Phosphorylation | Uniprot | |
| Y615 | Phosphorylation | Uniprot | |
| T648 | Phosphorylation | Uniprot | |
| K713 | Ubiquitination | Uniprot | |
| T882 | Phosphorylation | Uniprot | |
| Y883 | Phosphorylation | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| K894 | Ubiquitination | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| S904 | Phosphorylation | Uniprot | |
| S933 | Phosphorylation | Uniprot | |
| S936 | Phosphorylation | Uniprot | |
| Y937 | Phosphorylation | Uniprot | |
| S956 | Phosphorylation | Uniprot | |
| K957 | Acetylation | Uniprot | |
| S993 | Phosphorylation | Uniprot | |
| Y1075 | Phosphorylation | Uniprot | |
| S1077 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| S1273 | Phosphorylation | Uniprot | |
| Y1340 | Phosphorylation | Uniprot | |
| S1350 | Phosphorylation | Uniprot | |
| T1351 | Phosphorylation | Uniprot | |
| S1524 | Phosphorylation | Uniprot | |
| Y1628 | Phosphorylation | Uniprot | |
| T1882 | Phosphorylation | Uniprot | |
| Y1883 | Phosphorylation | Uniprot | |
| Y1895 | Phosphorylation | Uniprot | |
| K1929 | Ubiquitination | Uniprot | |
| T1956 | Phosphorylation | Uniprot | |
| Y2069 | Phosphorylation | Uniprot | |
| S2077 | Phosphorylation | Uniprot | |
| S2082 | Phosphorylation | Uniprot | |
| S2087 | Phosphorylation | Uniprot | |
| S2096 | Phosphorylation | Uniprot | |
| S2099 | Phosphorylation | Uniprot |
Research Backgrounds
Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.
In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Enriched and co-localizes with GTPase CDC42 at the immunological synapse formed during T cell/antigen presenting cell cognate interaction. Translocates from the cytoplasm to the plasma membrane in response to chemokine CXCL12/SDF-1-alpha stimulation.
Expressed in peripheral blood mononuclear cells (PBMCs).
Interacts (via DHR-2 domain) with GTPase CDC42; the interaction activates CDC42 by exchanging GDP for GTP. The unphosphorylated form interacts (via DHR-2 domain) with LRCH1 (via LRR repeats); the interaction prevents the association between DOCK8 and CDC42. Interacts with CCDC88B.
The DHR-2 domain is necessary and sufficient for the GEF activity.
Belongs to the DOCK family.
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