DSN1 Antibody - #DF12962

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Product: DSN1 Antibody
Catalog: DF12962
Description: Rabbit polyclonal antibody to DSN1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: Q9H410
RRID: AB_2845923

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Dog(90%)
Clonality:
Polyclonal
Specificity:
DSN1 Antibody detects endogenous levels of total DSN1.
RRID:
AB_2845923
Cite Format: Affinity Biosciences Cat# DF12962, RRID:AB_2845923.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C20orf172; Chromosome 20 open reading frame 172; dJ469A13.2; DSN1; DSN1, MIND kinetochore complex component, homolog; DSN1, MIND kinetochore complex component, homolog (S. cerevisiae); DSN1, MIS12 kinetochore complex component; DSN1_HUMAN; FLJ13346; hKNL-3; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore null 3 homolog; Kinetochore-associated protein DSN1 homolog; KNL3; MGC32987; MIS13;

Immunogens

Immunogen:
Uniprot:

Q9H410(DSN1_HUMAN) >>Visit HPA database.

Gene(ID):
DSN1(79980)
Sequence:
MTSVTRSEIIDEKGPVMSKTHDHQLESSLSPVEVFAKTSASLEMNQGVSEERIHLGSSPKKGGNCDLSHQERLQSKSLHLSPQEQSASYQDRRQSWRRASMKETNRRKSLHPIHQGITELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFSLESFRAKASSLSEELKHFADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKFSLERQTWDQLLLHYQQEAKEILSRGSTEAKITEVKVEPMTYLGSSQNEVLNTKPDYQKILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVSVQLGKRSMQQLDPSPARKLLKLQLQNPPAIHGSGSGSCQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
90
Pig
82
Horse
73
Bovine
64
Sheep
64
Rabbit
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H410 As Substrate

Site PTM Type Enzyme
K13 Sumoylation
T20 Phosphorylation
S27 Phosphorylation
S28 Phosphorylation
S30 Phosphorylation
S41 Phosphorylation
S49 Phosphorylation
S57 Phosphorylation
S58 Phosphorylation
K61 Sumoylation
S68 Phosphorylation
S75 Phosphorylation
K76 Sumoylation
K76 Ubiquitination
S77 Phosphorylation
S81 Phosphorylation
S86 Phosphorylation
S88 Phosphorylation
S95 Phosphorylation
S100 Phosphorylation Q96GD4 (AURKB)
K108 Sumoylation
K108 Ubiquitination
S109 Phosphorylation Q96GD4 (AURKB)
T118 Phosphorylation
S123 Phosphorylation
S125 Phosphorylation
S131 Phosphorylation
K132 Ubiquitination
K157 Sumoylation
K157 Ubiquitination
S163 Phosphorylation
K167 Sumoylation
K167 Ubiquitination
S170 Phosphorylation
K176 Ubiquitination
K190 Ubiquitination
K212 Ubiquitination
K217 Ubiquitination
S219 Phosphorylation
K237 Ubiquitination
S244 Phosphorylation
T245 Phosphorylation
K248 Ubiquitination
K253 Sumoylation
K253 Ubiquitination
K271 Ubiquitination
K276 Ubiquitination
K322 Ubiquitination
S331 Phosphorylation
K338 Sumoylation
K338 Ubiquitination
S350 Phosphorylation

Research Backgrounds

Function:

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Associated with the kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with KNL1, CBX3 and CBX5. Interacts with KNSTRN.

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