ECD Antibody - #DF12968
| Product: | ECD Antibody |
| Catalog: | DF12968 |
| Description: | Rabbit polyclonal antibody to ECD |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 73 kDa; 73kD(Calculated). |
| Uniprot: | O95905 |
| RRID: | AB_2845929 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12968, RRID:AB_2845929.
Fold/Unfold
ecd; ECD_HUMAN; Ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; SGT 1; SGT1 protein; Suppressor of GCR2;
Immunogens
Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers.
- O95905 ECD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95905 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| Y448 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S505 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S518 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S534 | Phosphorylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| S561 | Phosphorylation | Uniprot |
Research Backgrounds
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.
Cytoplasm. Nucleus.
Note: Predominantly is located in the cytoplasm.
Highly expressed in muscle and heart. Over-expressed in pancreatic and breast cancers.
Interacts with TP53, MDM2, TXNIP. Interacts (phosphorylated) with PIH1D1. Interacts with RUVBL1 mediating the PIH1D1-independent association with the R2TP complex. Interacts with RB1, RBL1 and RBL2; ECD competes with E2F1 for binding to hypophospshorylated RB1. Interacts with EP300.
Belongs to the ECD family.
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