EHBP1 Antibody - #DF12971

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Product: EHBP1 Antibody
Catalog: DF12971
Description: Rabbit polyclonal antibody to EHBP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 140 kDa; 140kD(Calculated).
Uniprot: Q8NDI1
RRID: AB_2845932

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
EHBP1 Antibody detects endogenous levels of total EHBP1.
RRID:
AB_2845932
Cite Format: Affinity Biosciences Cat# DF12971, RRID:AB_2845932.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EH domain binding protein 1; NACSIN;

Immunogens

Immunogen:
Uniprot:

Q8NDI1(EHBP1_HUMAN) >>Visit HPA database.

Gene(ID):
EHBP1(23301)
Sequence:
MASVWKRLQRVGKHASKFQFVASYQELMVECTKKWQPDKLVVVWTRRSRRKSSKAHSWQPGIKNPYRGVVVWPVPENIEITVTLFKDPHAEEFEDKEWTFVIENESPSGRRKALATSSINMKQYASPMPTQTDVKLKFKPLSKKVVSAALQFSLSCIFLREGKATDEDMQSLASLMSMKQADIGNLDDFEEDNEDDDENRVNQEEKAAKITEIVNQLNALSSLDEDQDDCIKQANMRSAKSASSSEELINKLNFLDEAEKDLATVNSNPFDDPDAAELNPFGDPDSEEPITETASPRKTEDSFYNNSYNPFKEVQTPQYLNPFDEPEAFVTIKDSPPQSTKRKNIRPVDMSKYLYADSSKTEEEELDESNPFYEPKSTPPPNNLVNPVQELETERRVKRKAPAPPVLSPKTGVLNENTVSAGKDLSTSPKPSPIPSPVLGRKPNASQSLLVWCKEVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAYDGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPELQQPISGAVDFLSQDDSVFVNDSGVGESESEHQTPDDHLSPSTASPYCRRTKSDTEPQKSQQSSGRTSGSDDPGICSNTDSTQAQVLLGKKRLLKAETLELSDLYVSDKKKDMSPPFICEETDEQKLQTLDIGSNLEKEKLENSRSLECRSDPESPIKKTSLSPTSKLGYSYSRDLDLAKKKHASLRQTESDPDADRTTLNHADHSSKIVQHRLLSRQEELKERARVLLEQARRDAALKAGNKHNTNTATPFCNRQLSDQQDEERRRQLRERARQLIAEARSGVKMSELPSYGEMAAEKLKERSKASGDENDNIEIDTNEEIPEGFVVGGGDELTNLENDLDTPEQNSKLVDLKLKKLLEVQPQVANSPSSAAQKAVTESSEQDMKSGTEDLRTERLQKTTERFRNPVVFSKDSTVRKTQLQSFSQYIENRPEMKRQRSIQEDTKKGNEEKAAITETQRKPSEDEVLNKGFKDTSQYVVGELAALENEQKQIDTRAALVEKRLRYLMDTGRNTEEEEAMMQEWFMLVNKKNALIRRMNQLSLLEKEHDLERRYELLNRELRAMLAIEDWQKTEAQKRREQLLLDELVALVNKRDALVRDLDAQEKQAEEEDEHLERTLEQNKGKMAKKEEKCVLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
75
Pig
56
Sheep
56
Xenopus
56
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NDI1 As Substrate

Site PTM Type Enzyme
K33 Acetylation
K34 Acetylation
S106 Phosphorylation
K163 Ubiquitination
T165 Phosphorylation
S171 Phosphorylation
S174 Phosphorylation
S177 Phosphorylation
K206 Ubiquitination
T291 Phosphorylation
T293 Phosphorylation
T299 Phosphorylation
S302 Phosphorylation
Y304 Phosphorylation
S307 Phosphorylation
Y319 Phosphorylation
T331 Phosphorylation
S335 Phosphorylation
Y355 Phosphorylation
S358 Phosphorylation
S369 Phosphorylation
T378 Phosphorylation
S408 Phosphorylation
S420 Phosphorylation
S426 Phosphorylation
T427 Phosphorylation
S428 Phosphorylation
S432 Phosphorylation
S436 Phosphorylation
S577 Phosphorylation
S578 Phosphorylation
Y585 Phosphorylation
S589 Phosphorylation
K592 Ubiquitination
T647 Phosphorylation
S649 Phosphorylation
T663 Phosphorylation
S666 Phosphorylation
T694 Phosphorylation
S698 Phosphorylation
Y701 Phosphorylation
S710 Phosphorylation
T718 Phosphorylation
S730 Phosphorylation
S742 Phosphorylation
S747 Phosphorylation
S751 Phosphorylation
T756 Phosphorylation
S759 Phosphorylation
K763 Ubiquitination
Y766 Phosphorylation
S767 Phosphorylation
Y768 Phosphorylation
S769 Phosphorylation
S781 Phosphorylation
S787 Phosphorylation
S812 Phosphorylation
T846 Phosphorylation
S854 Phosphorylation
S887 Phosphorylation
Y888 Phosphorylation
S964 Phosphorylation
S967 Phosphorylation
T974 Phosphorylation
S976 Phosphorylation
S977 Phosphorylation
T1011 Phosphorylation
S1019 Phosphorylation
Y1023 Phosphorylation
S1035 Phosphorylation
T1040 Phosphorylation
T1053 Phosphorylation
S1058 Phosphorylation
T1070 Phosphorylation
K1097 Ubiquitination
Y1101 Phosphorylation
K1188 Ubiquitination

Research Backgrounds

Function:

May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking. Required for perinuclear sorting and insulin-regulated recycling of SLC2A4/GLUT4 in adipocytes (By similarity).

PTMs:

Prenylated (Probable). Farnelysation (predominant) and geranylgeranylation has been observed in vitro.

Subcellular Location:

Cytoplasm. Membrane. Endosome.
Note: Mostly found in cytosol and plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with EHD1 (By similarity). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively.

Family&Domains:

The CAAX motif is a signal for prenylation and required for endosomal colocalization with Rab8 and Rab10.

The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound forms).

Restrictive clause

 

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