EIF4H Antibody - #DF12975

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Product: EIF4H Antibody
Catalog: DF12975
Description: Rabbit polyclonal antibody to EIF4H
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 27 kDa; 27kD(Calculated).
Uniprot: Q15056
RRID: AB_2845936

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
EIF4H Antibody detects endogenous levels of total EIF4H.
RRID:
AB_2845936
Cite Format: Affinity Biosciences Cat# DF12975, RRID:AB_2845936.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AU018978; D5Ertd355e; E430026L18Rik; Ef4h; eIF 4H; eIF-4H; EIF4H; Eukaryotic translation initiation factor 4H; IF4H_HUMAN; KIAA0038; WBSCR1; Williams Beuren syndrome chromosomal region 1 protein homolog; Williams Beuren syndrome chromosome region 1; Williams-Beuren syndrome chromosomal region 1 protein; Williams-Beuren syndrome chromosome region 1; WSCR1;

Immunogens

Immunogen:
Uniprot:

Q15056(IF4H_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4H(7458)
Expression:
Q15056 IF4H_HUMAN:

The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.

Sequence:
MADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKDLSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKEALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKGGPDDRGMGSSRESRGGWDSRDDFNSGFRDDFLGGRGGSRPGDRRTGPPMGSRFRDGPPLRGSNMDFREPTEEERAQRPRLQLKPRTVATPLNQVANPNSAIFGGARPREEVVQKEQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15056 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T6 Phosphorylation
Y7 Phosphorylation
R10 Methylation
Y12 Phosphorylation
S13 Phosphorylation
S14 Phosphorylation
R19 Methylation
S21 Phosphorylation
R22 Methylation
S24 Phosphorylation
S30 Phosphorylation
R31 Methylation
S32 Phosphorylation
K34 Ubiquitination
Y42 Phosphorylation
T43 Phosphorylation
Y45 Phosphorylation
R68 Methylation
S69 Phosphorylation
K80 Acetylation
K80 Ubiquitination
Y86 Phosphorylation
S94 Phosphorylation
T100 Phosphorylation
Y101 Phosphorylation
R109 Methylation
S110 Phosphorylation
K120 Ubiquitination
K123 Ubiquitination
K130 Methylation
R136 Methylation
R145 Methylation
R166 Methylation
R170 Methylation
R174 Methylation
R175 Methylation
S182 Phosphorylation
R183 Methylation
R185 Methylation
R191 Methylation
S193 Phosphorylation
R198 Methylation
T201 Phosphorylation
K214 Acetylation
K214 Sumoylation
T217 Phosphorylation
T220 Phosphorylation
S230 Phosphorylation
R237 Methylation
R239 Methylation
K245 Acetylation
K245 Ubiquitination

Research Backgrounds

Function:

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.

Subcellular Location:

Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.

Subunit Structure:

(Microbial infection) Interacts with HHV-1 Vhs.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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