Product: ERGIC1 Antibody
Catalog: DF12981
Description: Rabbit polyclonal antibody to ERGIC1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 32 kDa; 33kD(Calculated).
Uniprot: Q969X5
RRID: AB_2845942

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
ERGIC1 Antibody detects endogenous levels of total ERGIC1.
RRID:
AB_2845942
Cite Format: Affinity Biosciences Cat# DF12981, RRID:AB_2845942.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

endoplasmic reticulum-golgi intermediate compartment 32 kDa protein; Endoplasmic reticulum-Golgi intermediate compartment protein 1; ER-Golgi intermediate compartment 32 kDa protein; ERGI1_HUMAN; ERGIC-32; ergic1; ERGIC32; KIAA1181; NET24;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPFDFRRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDDPDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQFSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSNPLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
91
Chicken
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969X5 As Substrate

Site PTM Type Enzyme
Y11 Phosphorylation
N74 N-Glycosylation
Y187 Phosphorylation
K190 Ubiquitination
Y196 Phosphorylation
K199 Ubiquitination
Y209 Phosphorylation
Y216 Phosphorylation
Y219 Phosphorylation
S236 Phosphorylation
K241 Ubiquitination
K288 Ubiquitination

Research Backgrounds

Function:

Possible role in transport between endoplasmic reticulum and Golgi.

PTMs:

N-glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein.
Note: Cycles between the endoplasmic reticulum and the Golgi.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ERGIC3/ERV46.

Family&Domains:

Belongs to the ERGIC family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.