EYA3 Antibody - #DF12994

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Product: EYA3 Antibody
Catalog: DF12994
Description: Rabbit polyclonal antibody to EYA3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 66 kDa; 63kD(Calculated).
Uniprot: Q99504
RRID: AB_2845955

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
EYA3 Antibody detects endogenous levels of total EYA3.
RRID:
AB_2845955
Cite Format: Affinity Biosciences Cat# DF12994, RRID:AB_2845955.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI844637; DKFZp686C132; EYA3; EYA3_HUMAN; Eyes absent 3; Eyes absent 3 homolog (Drosophila); Eyes absent homolog 3; OTTMUSP00000010370; RP11-460I13.4; RP23-442A20.1;

Immunogens

Immunogen:
Uniprot:

Q99504(EYA3_HUMAN) >>Visit HPA database.

Gene(ID):
EYA3(2140)
Sequence:
MEEEQDLPEQPVKKAKMQESGEQTISQVSNPDVSDQKPETSSLASNLPMSEEIMTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFGALWPGMKPESGLIQTPSPSQHSVLTCTTGLTTSQPSPAHYSYPIQASSTNASLISTSSTIANIPAAAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSSGDPSTSPSLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDSELERVFLWDLDETIIIFHSLLTGSYAQKYGKDPTVVIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGSGGSGSHGSSVGVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSLLLIQSRKNCVNVLITTTQLVPALAKVLLYGLGEIFPIENIYSATKIGKESCFERIVSRFGKKVTYVVIGDGRDEEIAAKQHNMPFWRITNHGDLVSLHQALELDFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99504 As Substrate

Site PTM Type Enzyme
Phosphorylation
M1 Acetylation
K37 Sumoylation
S255 Phosphorylation
S256 Phosphorylation
S262 Phosphorylation
S264 Phosphorylation
S266 Phosphorylation Q13315 (ATM) , P24941 (CDK2)
T268 Phosphorylation P24941 (CDK2)
T269 Phosphorylation P24941 (CDK2)
S271 Phosphorylation P24941 (CDK2)
T274 Phosphorylation
T295 Phosphorylation
S297 Phosphorylation
K430 Methylation
K430 Ubiquitination
S438 Phosphorylation
S472 Phosphorylation
K515 Ubiquitination
K529 Ubiquitination
K546 Ubiquitination

Research Backgrounds

Function:

Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.

PTMs:

Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:19234442). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SIX1 and DACH1, and probably SIX2, SIX4, SIX5.

Family&Domains:

Belongs to the HAD-like hydrolase superfamily. EYA family.

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