FBXO27 Antibody - #DF13007

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Product: FBXO27 Antibody
Catalog: DF13007
Description: Rabbit polyclonal antibody to FBXO27
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: Q8NI29
RRID: AB_2845968

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(89%), Dog(100%)
Clonality:
Polyclonal
Specificity:
FBXO27 Antibody detects endogenous levels of total FBXO27.
RRID:
AB_2845968
Cite Format: Affinity Biosciences Cat# DF13007, RRID:AB_2845968.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

F box G domain protein 5; F box only protein 27; F box protein 27; F box protein FBG5; FBG5; Fbx27;

Immunogens

Immunogen:
Uniprot:

Q8NI29(FBX27_HUMAN) >>Visit HPA database.

Gene(ID):
FBXO27(126433)
Expression:
Q8NI29 FBX27_HUMAN:

Predominantly expressed in brain, heart and kidney. Expressed at lower levels in liver and lung.

Sequence:
MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRALVDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCGQEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHVFSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Rabbit
89
Xenopus
50
Pig
0
Horse
0
Bovine
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NI29 As Substrate

Site PTM Type Enzyme
T260 Phosphorylation

Research Backgrounds

Function:

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides.

Tissue Specificity:

Predominantly expressed in brain, heart and kidney. Expressed at lower levels in liver and lung.

Subunit Structure:

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.