Product: Phospho-PKD1/2/3/PKC mu (Ser738+Ser742) Antibody
Catalog: AF3444
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 101kD; 102kD,97kD,100kD(Calculated).
Uniprot: Q15139 | Q9BZL6 | O94806
RRID: AB_2834886

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PKD1/2/3/PKC mu (Ser738+Ser742) Antibody detects endogenous levels of PKD1/2/3/PKC mu only when phosphorylated at Serine 738+Serine 742.
RRID:
AB_2834886
Cite Format: Affinity Biosciences Cat# AF3444, RRID:AB_2834886.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KPCD1_HUMAN; nPKC D1; nPKC mu; nPKC-D1; nPKC-mu; nPKCD1; nPKCmu; PKC; PKC MU; PKCM; PKCmu; PKD 1; PKD; PKD1; PRKCM; PRKD 1; Prkd1; Protein kinase C mu; Protein kinase C mu type; Protein kinase D; Protein kinase D1; Serine/threonine protein kinase D1; Serine/threonine-protein kinase D1; DKFZp586E0820; HSPC187; KPCD2_HUMAN; nPKC D2; nPKC-D2; PKD2; PRKD 2; Prkd2; Protein kinase D2; Serine/threonine protein kinase D2; Serine/threonine-protein kinase D2; EPK 2; EPK2; KPCD3_HUMAN; nPKC nu; nPKC-nu; nPKCnu; nu; PKCnu; PKD 3; PKD3; PRK D3; PRKCN; PRKD 3; Prkd3; Protein kinase C; Protein kinase C nu; Protein kinase C nu type; Protein kinase D3; Protein kinase EPK 2; Protein kinase EPK2; Serine threonine protein kinase; Serine/threonine protein kinase D3; Serine/threonine-protein kinase D3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BZL6 KPCD2_HUMAN:

Widely expressed.

O94806 KPCD3_HUMAN:

Ubiquitous.

Description:
PKD3 a CAMK kinase of the PKD family. An important component of signaling pathways downstream from novel PKC enzymes after B-cell receptor engagement.
Sequence:
MSAPPVLRPPSPLLPVAAAAAAAAAALVPGSGPGPAPFLAPVAAPVGGISFHLQIGLSREPVLLLQDSSGDYSLAHVREMACSIVDQKFPECGFYGMYDKILLFRHDPTSENILQLVKAASDIQEGDLIEVVLSASATFEDFQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRLSNVSLTGVSTIRTSSAELSTSAPDEPLLQKSPSESFIGREKRSNSQSYIGRPIHLDKILMSKVKVPHTFVIHSYTRPTVCQYCKKLLKGLFRQGLQCKDCRFNCHKRCAPKVPNNCLGEVTINGDLLSPGAESDVVMEEGSDDNDSERNSGLMDDMEEAMVQDAEMAMAECQNDSGEMQDPDPDHEDANRTISPSTSNNIPLMRVVQSVKHTKRKSSTVMKEGWMVHYTSKDTLRKRHYWRLDSKCITLFQNDTGSRYYKEIPLSEILSLEPVKTSALIPNGANPHCFEITTANVVYYVGENVVNPSSPSPNNSVLTSGVGADVARMWEIAIQHALMPVIPKGSSVGTGTNLHRDISVSISVSNCQIQENVDISTVYQIFPDEVLGSGQFGIVYGGKHRKTGRDVAIKIIDKLRFPTKQESQLRNEVAILQNLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPEHITKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDIHDQIQNAAFMYPPNPWKEISHEAIDLINNLLQVKMRKRYSVDKTLSHPWLQDYQTWLDLRELECKIGERYITHESDDLRWEKYAGEQGLQYPTHLINPSASHSDTPETEETEMKALGERVSIL

MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL

MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSADEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDIDNNDINSDSSRGLDDTEEPSPPEDKMFFLDPSDLDVERDEEAVKTISPSTSNNIPLMRVVQSIKHTKRKSSTMVKEGWMVHYTSRDNLRKRHYWRLDSKCLTLFQNESGSKYYKEIPLSEILRISSPRDFTNISQGSNPHCFEIITDTMVYFVGENNGDSSHNPVLAATGVGLDVAQSWEKAIRQALMPVTPQASVCTSPGQGKDHKDLSTSISVSNCQIQENVDISTVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFMVTQILVALRNLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPNPWREISGEAIDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRIGERYITHESDDARWEIHAYTHNLVYPKHFIMAPNPDDMEEDP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15139/Q9BZL6/O94806 As Substrate

Site PTM Type Enzyme
Y95 Phosphorylation P12931 (SRC)
S205 Phosphorylation Q15139 (PRKD1) , P17612 (PRKACA) , P98161 (PKD1)
S208 Phosphorylation O15264 (MAPK13)
T210 Phosphorylation
T217 Phosphorylation
S219 Phosphorylation
S223 Phosphorylation
T224 Phosphorylation
S225 Phosphorylation
S235 Phosphorylation
S237 Phosphorylation
S239 Phosphorylation
S247 Phosphorylation
S249 Phosphorylation O15264 (MAPK13)
K292 Ubiquitination
S345 Phosphorylation
S379 Phosphorylation
T395 Phosphorylation
S397 Phosphorylation O15264 (MAPK13)
T400 Phosphorylation
S401 Phosphorylation O15264 (MAPK13)
S412 Phosphorylation Q05655 (PRKCD)
S421 Phosphorylation P17612 (PRKACA)
T422 Phosphorylation
Y432 Phosphorylation A0A173G4P4 (Abl fusion) , P12931 (SRC) , P00519 (ABL1)
Y443 Phosphorylation
S448 Phosphorylation
S460 Phosphorylation
Y463 Phosphorylation P12931 (SRC) , A0A173G4P4 (Abl fusion) , P00519 (ABL1)
S473 Phosphorylation
Y502 Phosphorylation P12931 (SRC) , A0A173G4P4 (Abl fusion) , P00519 (ABL1)
S548 Phosphorylation
S549 Phosphorylation
K612 Acetylation
K622 Ubiquitination
K675 Ubiquitination
K708 Ubiquitination
K724 Ubiquitination
K737 Ubiquitination
S738 Phosphorylation O94806 (PRKD3) , P24723 (PRKCH) , Q15139 (PRKD1) , P17252 (PRKCA) , Q05655 (PRKCD) , Q02156 (PRKCE) , Q05513 (PRKCZ)
S742 Phosphorylation O94806 (PRKD3) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P24723 (PRKCH) , Q15139 (PRKD1) , O15264 (MAPK13) , Q02156 (PRKCE) , P17252 (PRKCA)
T746 Phosphorylation
Y749 Phosphorylation
S890 Phosphorylation
S892 Phosphorylation
S910 Phosphorylation Q15139 (PRKD1) , P98161 (PKD1)
Site PTM Type Enzyme
K80 Ubiquitination
Y87 Phosphorylation
K92 Ubiquitination
K97 Ubiquitination
T101 Phosphorylation
K178 Ubiquitination
S183 Phosphorylation
S189 Phosphorylation
S197 Phosphorylation
S198 Phosphorylation
T199 Phosphorylation
S200 Phosphorylation
S203 Phosphorylation
S206 Phosphorylation
T211 Phosphorylation
S212 Phosphorylation
S214 Phosphorylation
C217 S-Nitrosylation
S225 Phosphorylation
T227 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
S238 Phosphorylation
S239 Phosphorylation
S241 Phosphorylation
S242 Phosphorylation
S244 Phosphorylation P48730 (CSNK1D) , P49674 (CSNK1E)
S245 Phosphorylation
Y246 Phosphorylation
K255 Ubiquitination
K260 Ubiquitination
Y272 Phosphorylation
K286 Ubiquitination
S355 Phosphorylation
S362 Phosphorylation
S374 Phosphorylation
S375 Phosphorylation
Y378 Phosphorylation
S387 Phosphorylation
T391 Phosphorylation
T392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation
T398 Phosphorylation
T412 Phosphorylation
K424 Ubiquitination
Y438 Phosphorylation P29597 (TYK2)
S518 Phosphorylation
S529 Phosphorylation
K572 Acetylation
K580 Acetylation
K580 Ubiquitination
K584 Acetylation
K590 Ubiquitination
T619 Phosphorylation
K643 Ubiquitination
K676 Ubiquitination
K692 Ubiquitination
K705 Ubiquitination
S706 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA) , P24723 (PRKCH)
S710 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA) , P24723 (PRKCH)
T714 Phosphorylation
Y717 Phosphorylation
S876 Phosphorylation P24723 (PRKCH) , Q9BZL6 (PRKD2) , P17252 (PRKCA) , Q13563 (PKD2) , Q02156 (PRKCE)
Site PTM Type Enzyme
S2 Phosphorylation
S6 Phosphorylation
S9 Phosphorylation
S13 Phosphorylation
S27 Phosphorylation
S30 Phosphorylation
S31 Phosphorylation
T34 Phosphorylation
S37 Phosphorylation
S41 Phosphorylation
S44 Phosphorylation
S46 Phosphorylation
S213 Phosphorylation
S216 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
K293 Ubiquitination
S315 Phosphorylation
T360 Phosphorylation
S364 Phosphorylation
T389 Phosphorylation
S391 Phosphorylation
S395 Phosphorylation
S406 Phosphorylation
K408 Ubiquitination
S415 Phosphorylation
T446 Phosphorylation
Y456 Phosphorylation
S463 Phosphorylation
T535 Phosphorylation
S543 Phosphorylation
K605 Ubiquitination
K615 Ubiquitination
K730 Ubiquitination
S731 Phosphorylation P17252 (PRKCA)
S735 Phosphorylation
T739 Phosphorylation
Y742 Phosphorylation
K816 Ubiquitination

PTMs - Q15139/Q9BZL6/O94806 As Enzyme

Substrate Site Source
O00206 (TLR4) S790 Uniprot
O14558 (HSPB6) S16 Uniprot
O15273 (TCAP) S157 Uniprot
O15273 (TCAP) S161 Uniprot
O43865 (AHCYL1) S68 Uniprot
O60602 (TLR5) S805 Uniprot
O75628 (REM1) S18 Uniprot
O95863 (SNAI1) S11 Uniprot
O96013 (PAK4) S99 Uniprot
P00533 (EGFR) T678 Uniprot
P00533 (EGFR) T693 Uniprot
P02686-5 (MBP) S162 Uniprot
P04792 (HSPB1) S82 Uniprot
P05412 (JUN) S63 Uniprot
P05549 (TFAP2A) S258 Uniprot
P05549 (TFAP2A) S326 Uniprot
P16144-2 (ITGB4) T1736 Uniprot
P16220 (CREB1) S98 Uniprot
P16220 (CREB1) S133 Uniprot
P19174-1 (PLCG1) S1248 Uniprot
P19429 (TNNI3) S23 Uniprot
P19429 (TNNI3) S24 Uniprot
P22059 (OSBP) S240 Uniprot
P23528 (CFL1) S3 Uniprot
P27986 (PIK3R1) S652 Uniprot
P31749 (AKT1) T308 Uniprot
P31749 (AKT1) S473 Uniprot
P35222 (CTNNB1) T112 Uniprot
P35222 (CTNNB1) T120 Uniprot
P43405 (SYK) S291 Uniprot
P48426 (PIP4K2A) T376 Uniprot
P50552 (VASP) S157 Uniprot
P50552 (VASP) S322 Uniprot
P53367-2 (ARFIP1) S100 Uniprot
P53367 (ARFIP1) S132 Uniprot
Q13671 (RIN1) S292 Uniprot
Q13671 (RIN1) S351 Uniprot
Q13936 (CACNA1C) T1928 Uniprot
Q13936 (CACNA1C) S1981 Uniprot
Q14247 (CTTN) S298 Uniprot
Q14247 (CTTN) S348 Uniprot
Q14896 (MYBPC3) S304 Uniprot
Q15139 (PRKD1) S205 Uniprot
Q15139 (PRKD1) S738 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q15139 (PRKD1) S910 Uniprot
Q15276 (RABEP1) S407 Uniprot
Q7KZI7 (MARK2) S400 Uniprot
Q8NER1 (TRPV1) S117 Uniprot
Q8WUI4 (HDAC7) S155 Uniprot
Q8WUI4-5 (HDAC7) S194 Uniprot
Q8WUI4-5 (HDAC7) S220 Uniprot
Q8WUI4 (HDAC7) S358 Uniprot
Q8WUI4-5 (HDAC7) S397 Uniprot
Q8WUI4 (HDAC7) S486 Uniprot
Q8WYL5 (SSH1) S402 Uniprot
Q8WYL5 (SSH1) S937 Uniprot
Q8WYL5 (SSH1) S978 Uniprot
Q92918 (MAP4K1) S171 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q92934 (BAD) S118 Uniprot
Q96A00 (PPP1R14A) T38 Uniprot
Q96QB1 (DLC1) S1244 Uniprot
Q99683 (MAP3K5) T838 Uniprot
Q99814 (EPAS1) T324 Uniprot
Q9BST9-2 (RTKN) S435 Uniprot
Q9BST9 (RTKN) S448 Uniprot
Q9NRA0 (SPHK2) S419 Uniprot
Q9NRA0 (SPHK2) S421 Uniprot
Q9UBF8 (PI4KB) S294 Uniprot
Q9ULH0-1 (KIDINS220) S918 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot
Q9UQL6-3 (HDAC5) S260 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Q9UQL6-3 (HDAC5) S499 Uniprot
Q9Y3E5 (PTRH2) S5 Uniprot
Q9Y3E5 (PTRH2) S87 Uniprot
Q9Y5P4 (COL4A3BP) S132 Uniprot
Substrate Site Source
P16144-2 (ITGB4) T1736 Uniprot
P17181 (IFNAR1) S535 Uniprot
P50552 (VASP) S157 Uniprot
P50552 (VASP) S239 Uniprot
P50552 (VASP) S322 Uniprot
Q01105-2 (SET) S171 Uniprot
Q13563 (PKD2) S801 Uniprot
Q13671 (RIN1) S292 Uniprot
Q7KZI7 (MARK2) S400 Uniprot
Q8WYL5 (SSH1) S937 Uniprot
Q8WYL5 (SSH1) S978 Uniprot
Q99828 (CIB1) S78 Uniprot
Q9BST9 (RTKN) S448 Uniprot
Q9BZL6 (PRKD2) S876 Uniprot
Q9UBF8-2 (PI4KB) S294 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Substrate Site Source
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
Q04206 (RELA) S276 Uniprot
Q04206 (RELA) S536 Uniprot
Q15139 (PRKD1) S738 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q9BST9 (RTKN) S448 Uniprot
Q9NRA0 (SPHK2) S419 Uniprot
Q9NRA0 (SPHK2) S421 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Q9Y2X7 (GIT1) S46 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenetic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor. Plays a role in activated KRAS-mediated stabilization of ZNF304 in colorectal cancer (CRC) cells. Regulates nuclear translocation of transcription factor TFEB in macrophages upon live S.enterica infection (By similarity).

PTMs:

Phosphorylated at Ser-397 and Ser-401 by MAPK13 during regulation of insulin secretion in pancreatic beta cells. Phosphorylated by DAPK1. Phosphorylated at Tyr-95 and by ABL at Tyr-463, which primes the kinase in response to oxidative stress, and promotes a second step activating phosphorylation at Ser-738/Ser-742 by PKRD. Phosphorylated on Ser-910 upon S.enterica infection in macrophages (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane. Golgi apparatus>trans-Golgi network.
Note: Translocation to the cell membrane is required for kinase activation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with MAPK13. Interacts with DAPK1 in an oxidative stress-regulated manner. Interacts with USP28; the interaction induces phosphorylation of USP28 and activated KRAS-mediated stabilization of ZNF304. Interacts with AKAP13 (via C-terminal domain) (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Function:

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression (By similarity). In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens (By similarity). In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion.

PTMs:

Phosphorylation of Ser-876 correlates with the activation status of the kinase. Ser-706 or/and Ser-710 are probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-706 and Ser-710 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress. Phosphorylated at Tyr-717 by ABL1 specifically in response to oxidative stress; requires prior phosphorylation at Ser-706 or/and Ser-710.

Subcellular Location:

Cytoplasm. Cell membrane. Nucleus. Golgi apparatus>trans-Golgi network.
Note: Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts (via C-terminus) with LCK. Interacts (via N-terminal AP-rich region) with CIB1 isoform 2. Interacts (via N-terminus and zing-finger domain 1 and 2) with PRKCD in response to oxidative stress; the interaction is independent of PRKD2 tyrosine phosphorylation.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Function:

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).

Subcellular Location:

Cytoplasm. Membrane.
Note: Translocation to the cell membrane is required for kinase activation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

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