FERMT1 Antibody - #DF13012
| Product: | FERMT1 Antibody |
| Catalog: | DF13012 |
| Description: | Rabbit polyclonal antibody to FERMT1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 70 kDa; 77kD(Calculated). |
| Uniprot: | Q9BQL6 |
| RRID: | AB_2845973 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13012, RRID:AB_2845973.
Fold/Unfold
C20orf42; Chromosome 20 open reading frame 42; DTGCU 2; DTGCU2; FERM1_HUMAN; Fermitin family homolog 1; Fermitin family member 1; Fermt1; FLJ20116; FLJ23423; KIND 1; KIND1; Kinderlin; Kindlerin; Kindlin 1; Kindlin syndrome protein; Kindlin-1; Kindlin1; Unc 112 related protein 1; Unc-112-related protein 1; Unc112 related protein; UNC112A; URP 1; URP1;
Immunogens
Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes. Not detected in epidermal melanocytes and dermal fibroblasts.
- Q9BQL6 FERM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSSTDFTFASWELVVRVDHPNEEQQKDVTLRVSGDLHVGGVMLKLVEQINISQDWSDFALWWEQKHCWLLKTHWTLDKYGVQADAKLLFTPQHKMLRLRLPNLKMVRLRVSFSAVVFKAVSDICKILNIRRSEELSLLKPSGDYFKKKKKKDKNNKEPIIEDILNLESSPTASGSSVSPGLYSKTMTPIYDPINGTPASSTMTWFSDSPLTEQNCSILAFSQPPQSPEALADMYQPRSLVDKAKLNAGWLDSSRSLMEQGIQEDEQLLLRFKYYSFFDLNPKYDAVRINQLYEQARWAILLEEIDCTEEEMLIFAALQYHISKLSLSAETQDFAGESEVDEIEAALSNLEVTLEGGKADSLLEDITDIPKLADNLKLFRPKKLLPKAFKQYWFIFKDTSIAYFKNKELEQGEPLEKLNLRGCEVVPDVNVAGRKFGIKLLIPVADGMNEMYLRCDHENQYAQWMAACMLASKGKTMADSSYQPEVLNILSFLRMKNRNSASQVASSLENMDMNPECFVSPRCAKRHKSKQLAARILEAHQNVAQMPLVEAKLRFIQAWQSLPEFGLTYYLVRFKGSKKDDILGVSYNRLIKIDAATGIPVTTWRFTNIKQWNVNWETRQVVIEFDQNVFTAFTCLSADCKIVHEYIGGYIFLSTRSKDQNETLDEDLFHKLTGGQD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BQL6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K148 | Acetylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| Y392 | Phosphorylation | Uniprot | |
| K435 | Acetylation | Uniprot | |
| Y452 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot | |
| T663 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot |
Research Backgrounds
Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression.
Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Constituent of focal adhesions. Localized at the basal aspect of skin keratinocytes, close to the cell membrane. Colocalizes with filamentous actin. Upon TGFB1 treatment, it localizes to membrane ruffles.
Expressed in brain, skeletal muscle, kidney, colon, adrenal gland, prostate, and placenta. Weakly or not expressed in heart, thymus, spleen, liver, small intestine, bone marrow, lung and peripheral blood leukocytes. Overexpressed in some colon and lung tumors. In skin, it is localized within the epidermis and particularly in basal keratocytes. Not detected in epidermal melanocytes and dermal fibroblasts.
Interacts with the cytoplasmic domain of integrins ITGB1 and ITGB3.
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain. The FERM domain contains the subdomains F1, F2 and F3. It is preceded by a F0 domain with a ubiquitin-like fold. The F0 domain is required for integrin activation and for localization at focal adhesions.
Belongs to the kindlin family.
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