FGD5 Antibody - #DF13013

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Product: FGD5 Antibody
Catalog: DF13013
Description: Rabbit polyclonal antibody to FGD5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 160 kDa; 160kD(Calculated).
Uniprot: Q6ZNL6
RRID: AB_2845974

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
FGD5 Antibody detects endogenous levels of total FGD5.
RRID:
AB_2845974
Cite Format: Affinity Biosciences Cat# DF13013, RRID:AB_2845974.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FYVE, RhoGEF and PH domain containing protein 5; ZFYVE23; Zinc finger FYVE domain containing protein 23;

Immunogens

Immunogen:
Uniprot:

Q6ZNL6(FGD5_HUMAN) >>Visit HPA database.

Gene(ID):
FGD5(152273)
Expression:
Q6ZNL6 FGD5_HUMAN:

Expressed in endothelial cells (at protein level).

Sequence:
MFRGPKPPIAPKPRLTAPNEWRASVYLNDSLNKCSNGRLPCVDRGLDEGPRSIPKCSESETDEDYIVVPRVPLREDEPKDEGSVGNKALVSPESSAEEEEEREEGGEACGLEGTGAGEDSVAPAAPGAGALSREGEEGTDLALEDEGEGCADEPGTLEQVSRSEEEEKLVQPHRECSLEDSGPWAGEGVFQSDLLLPHIHGEDQEPPDTPGEAEEDDEEGCASTDPAGADEGSGPDRPTEDMGQDAEDTSEEPPEKEELAGVQEAETATDCPEVLEEGCEEATGVTGGEQVDLSEPPDHEKKTNQEVAAATLEDHAQDESAEESCQIVPFENDCMEDFVTSLTGSPYEFFPTESTSFCSESCSPLSESAKGLESEQAPKLGLRAEENPMVGALCGQCGSLQGGAAEGPAAPDVVVVLEEEALDDALANPYVMGVGLPGQAAPGEGGQAASDALGGYGSKEELNCEAEGGLVPADRKNTSTRVRPHSGKVAGYVPETVPEETGPEAGSSAPGIGGAAEEVGKTLLSLEGKPLEASRALPAKPRAFTLYPRSFSVEGREIPVSVYQEPEGSGLDDHRIKRKEDNLSLSCVIGSSGSFSQRNHLPSSGTSTPSSMVDIPPPFDLACITKKPITKSSPSLLIESDSPDKYKKKKSSFKRFLALTFKKKTENKLHVDVNVSSSRSSSESSYHGPSRILEVDRRSLSNSPQLKSRTGKLRASESPSSLIFYRDGKRKGVPFSRTVSRVESFEDRSRPPFLPLPLTKPRSISFPSADTSDYENIPAMNSDYENIQIPPRRPARAGAFTKLFEDQSRALSTANENDGYVDMSSFNAFESKQQSADQDAESAYTEPYKVCPISSAAPKEDLTSDEEQRSSEEEDSASRDPSVTHKVEGQSRALVIAQELLSSEKAYVEMLQHLNLDFHGAVMRALDDMDHEGRDTLAREELRQGLSELPAIHDLHQGILEELEERLSNWESQQKVADVFLAREQGFDHHATHILQFDRYLGLLSENCLHSPRLAAAVREFEQSVQGGSQTAKHRLLRVVQRLFQYQVLLTDYLNNLCPDSAEYDNTQGALSLISKVTDRANDSMEQGENLQKLVHIEHSVRGQGDLLQPGREFLKEGTLMKVTGKNRRPRHLFLMNDVLLYTYPQKDGKYRLKNTLAVANMKVSRPVMEKVPYALKIETSESCLMLSASSCAERDEWYGCLSRALPEDYKAQALAAFHHSVEIRERLGVSLGERPPTLVPVTHVMMCMNCGCDFSLTLRRHHCHACGKIVCRNCSRNKYPLKYLKDRMAKVCDGCFGELKKRGRAVPGLMRERPVSMSFPLSSPRFSGSAFSSVFQSINPSTFKKQKKVPSALTEVAASGEGSAISGYLSRCKRGKRHWKKLWFVIKGKVLYTYMASEDKVALESMPLLGFTIAPEKEEGSSEVGPIFHLYHKKTLFYSFKAEDTNSAQRWIEAMEDASVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Chicken
83
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6ZNL6 As Substrate

Site PTM Type Enzyme
S30 Phosphorylation
S52 Phosphorylation
S57 Phosphorylation
S59 Phosphorylation
Y65 Phosphorylation
T522 Phosphorylation
S552 Phosphorylation
S632 Phosphorylation
S633 Phosphorylation
S640 Phosphorylation
S642 Phosphorylation
Y646 Phosphorylation
K649 Acetylation
K650 Acetylation
K654 Acetylation
T660 Phosphorylation
S699 Phosphorylation
S703 Phosphorylation
S716 Phosphorylation
S718 Phosphorylation
K729 Acetylation
S740 Phosphorylation
S744 Phosphorylation
S749 Phosphorylation
T759 Phosphorylation
S765 Phosphorylation
T771 Phosphorylation
Y774 Phosphorylation
Y784 Phosphorylation
Y820 Phosphorylation
Y844 Phosphorylation
S870 Phosphorylation
S871 Phosphorylation
S878 Phosphorylation
K975 Ubiquitination
S1029 Phosphorylation
K1033 Ubiquitination
K1116 Ubiquitination
K1122 Ubiquitination
Y1142 Phosphorylation
K1154 Ubiquitination
T1156 Phosphorylation
K1163 Ubiquitination
Y1199 Phosphorylation
S1221 Phosphorylation
K1291 Acetylation
K1301 Methylation
K1301 Ubiquitination
S1317 Phosphorylation
S1367 Phosphorylation
S1371 Phosphorylation

Research Backgrounds

Function:

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane. Endoplasmic reticulum. Golgi apparatus. Early endosome.
Note: In peripheral membrane ruffles, colocolizes with F-actin. In confluent HUVECs, detected at cell-cell-contact sites where it colocalizes with vascular endothelial cadherin/CDH5.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in endothelial cells (at protein level).

References

1). Exosomes derived from dental pulp stem cells accelerate cutaneous wound healing by enhancing angiogenesis via the Cdc42/p38 MAPK pathway. International Journal of Molecular Medicine, 2022 (PubMed: 36321793) [IF=5.7]

Application: WB    Species: Human    Sample:

Figure 3 - Expression and function of the proteins identified by TMT-labeled quantitative proteomic in DPSC-Exos. (A) The number of differentially expressed proteins in DPSC-Exos compared with DPSCs was identified using TMT-labeled quantitative proteomics analysis. (B) Volcano map of differentially expressed proteins. (C) Differentially expressed proteins were categorized according to the biological process (Gene Ontology term) they were involved in; several of these were related to wound healing. (D) Kyoto Encyclopedia of Genes and Genomes pathway analysis of DPSC-Exos revealed that the enriched proteins involved in multiple signal transductions, including the MAPK signaling pathway. (E and F) The expression of Cdc42, p38 and p-p38 in HUVECs was higher following treatment with DPSC-Exos (n=3). *P

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