Product: GLOD4 Antibody
Catalog: DF13040
Description: Rabbit polyclonal antibody to GLOD4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: Q9HC38
RRID: AB_2846001

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%)
Clonality:
Polyclonal
Specificity:
GLOD4 Antibody detects endogenous levels of total GLOD4.
RRID:
AB_2846001
Cite Format: Affinity Biosciences Cat# DF13040, RRID:AB_2846001.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C17orf25; CGI 150; Glod4; GLOD4_HUMAN; Glyoxalase domain containing 4; glyoxalase domain containing protein 4; Glyoxalase domain-containing protein 4; HC71; my027;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9HC38 GLOD4_HUMAN:

Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung.

Sequence:
MAARRALHFVFKVGNRFQTARFYRDVLGMKVESCSVARLECSGAISAHCSDYTRITEDSFSKPYDGKWSKTMVGFGPEDDHFVAELTYNYGVGDYKLGNDFMGITLASSQAVSNARKLEWPLTEVAEGVFETEAPGGYKFYLQNRSLPQSDPVLKVTLAVSDLQKSLNYWCNLLGMKIYEKDEEKQRALLGYADNQCKLELQGVKGGVDHAAAFGRIAFSCPQKELPDLEDLMKRENQKILTPLVSLDTPGKATVQVVILADPDGHEICFVGDEAFRELSKMDPEGSKLLDDAMAADKSDEWFAKHNKPKASG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
89
Dog
78
Zebrafish
78
Rabbit
78
Bovine
67
Sheep
67
Xenopus
67
Chicken
67
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC38 As Substrate

Site PTM Type Enzyme
K117 Ubiquitination
K139 Ubiquitination
S146 Phosphorylation
K181 Acetylation
C197 S-Nitrosylation
K198 Ubiquitination
K205 Ubiquitination
C221 S-Nitrosylation
K224 Ubiquitination
K234 Acetylation
K234 Ubiquitination
K239 Ubiquitination
T242 Phosphorylation
S246 Phosphorylation
T249 Phosphorylation
S280 Phosphorylation
S287 Phosphorylation
K298 Ubiquitination
K305 Ubiquitination

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung.

Subunit Structure:

Interacts with NUDT9.

Family&Domains:

Belongs to the glyoxalase I family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.