GOLGA3 Antibody - #DF13049
(3)
| Product: | GOLGA3 Antibody |
| Catalog: | DF13049 |
| Description: | Rabbit polyclonal antibody to GOLGA3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Dog |
| Mol.Wt.: | 167 kDa; 167kD(Calculated). |
| Uniprot: | Q08378 |
| RRID: | AB_2846010 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(80%), Horse(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
GOLGA3 Antibody detects endogenous levels of total GOLGA3.
RRID:
AB_2846010
Cite Format: Affinity Biosciences Cat# DF13049, RRID:AB_2846010.
Cite Format: Affinity Biosciences Cat# DF13049, RRID:AB_2846010.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
GCP170; GOLGA 3; GOLGA3; Golgi autoantigen; Golgi complex associated protein of 170 kDa; Golgi complex-associated protein of 170 kD; Golgi membrane associated protein; Golgi peripheral membrane protein; Golgin 160; Golgin subfamily a 3; HGNC:4426; Male enhanced antigen 2; MEA2; SY2/SY10 protein;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Q08378 GOGA3_HUMAN:
Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney.
Sequence:
- Q08378 GOGA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGASAEQDGLQEDRSHSGPSSLPEAPLKPPGPLVPPDQQDKVQCAEVNRASTEGESPDGPGQGGLCQNGPTPPFPDPPSSLDPTTSPVGPDASPGVAGFHDNLRKSQGTSAEGSVRKEALQSLRLSLPMQETQLCSTDSPLPLEKEEQVRLQARKWLEEQLKQYRVKRQQERSSQPATKTRLFSTLDPELMLNPENLPRASTLAMTKEYSFLRTSVPRGPKVGSLGLPAHPREKKTSKSSKIRSLADYRTEDSNAGNSGGNVPAPDSTKGSLKQNRSSAASVVSEISLSPDTDDRLENTSLAGDSVSEVDGNDSDSSSYSSASTRGTYGILSKTVGTQDTPYMVNGQEIPADTLGQFPSIKDVLQAAAAEHQDQGQEVNGEVRSRRDSICSSVSLESSAAETQEEMLQVLKEKMRLEGQLEALSLEASQALKEKAELQAQLAALSTKLQAQVECSHSSQQRQDSLSSEVDTLKQSCWDLERAMTDLQNMLEAKNASLASSNNDLQVAEEQYQRLMAKVEDMQRSMLSKDNTVHDLRQQMTALQSQLQQVQLERTTLTSKLKASQAEISSLQSVRQWYQQQLALAQEARVRLQGEMAHIQVGQMTQAGLLEHLKLENVSLSQQLTETQHRSMKEKGRIAAQLQGIEADMLDQEAAFMQIQEAKTMVEEDLQRRLEEFEGERERLQRMADSAASLEQQLEQVKLTLLQRDQQLEALQQEHLDLMKQLTLTQEALQSREQSLDALQTHYDELQARLGELQGEAASREDTICLLQNEKIILEAALQAAKSGKEELDRGARRLEEGTEETSETLEKLREELAIKSGQVEHLQQETAALKKQMQKIKEQFLQQKVMVEAYRRDATSKDQLISELKATRKRLDSELKELRQELMQVHGEKRTAEAELSRLHREVAQVRQHMADLEGHLQSAQKERDEMETHLQSLQFDKEQMVAVTEANEALKKQIEELQQEARKAITEQKQKMRRLGSDLTSAQKEMKTKHKAYENAVGILSRRLQEALAAKEAADAELGQLRAQGGSSDSSLALHERIQALEAELQAVSHSKTLLEKELQEVIALTSQELEESREKVLELEDELQESRGFRKKIKRLEESNKKLALELEHEKGKLTGLGQSNAALREHNSILETALAKREADLVQLNLQVQAVLQRKEEEDRQMKHLVQALQASLEKEKEKVNSLKEQVAAAKVEAGHNRRHFKAASLELSEVKKELQAKEHLVQKLQAEADDLQIREGKHSQEIAQFQAELAEARAQLQLLQKQLDEQLSKQPVGNQEMENLKWEVDQKEREIQSLKQQLDLTEQQGRKELEGLQQLLQNVKSELEMAQEDLSMTQKDKFMLQAKVSELKNNMKTLLQQNQQLKLDLRRGAAKTRKEPKGEASSSNPATPIKIPDCPVPASLLEELLRPPPAVSKEPLKNLNSCLQQLKQEMDSLQRQMEEHALTVHESLSSWTPLEPATASPVPPGGHAGPRGDPQRHSQSRASKEGPGE
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
80
Horse
80
Dog
80
Bovine
70
Sheep
70
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q08378 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| T186 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| Y210 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| Y249 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| Y343 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| Y512 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| T556 | Phosphorylation | Uniprot | |
| K562 | Methylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S739 | Phosphorylation | Uniprot | |
| T872 | Phosphorylation | Uniprot | |
| S878 | Phosphorylation | Uniprot | |
| T950 | Phosphorylation | Uniprot | |
| S983 | Phosphorylation | Uniprot | |
| T986 | Phosphorylation | Uniprot | |
| S1127 | Phosphorylation | Uniprot | |
| S1180 | Phosphorylation | Uniprot | |
| S1213 | Phosphorylation | Uniprot | |
| K1304 | Methylation | Uniprot | |
| S1354 | Phosphorylation | Uniprot | |
| S1392 | Phosphorylation | Uniprot | |
| T1396 | Phosphorylation | Uniprot | |
| K1426 | Acetylation | Uniprot | |
| S1430 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Golgi auto-antigen; probably involved in maintaining Golgi structure.
PTMs:
Cleaved by caspases in apoptotic cells.
Subcellular Location:
Cytoplasm. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein.
Tissue Specificity:
Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney.
Subunit Structure:
Homodimer. Interacts with GOLGA7. Isoform 1 interacts with GOPC while isoform 3 does not.
Family&Domains:
Extended rod-like protein with coiled-coil domains.
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