HMBOX1 Antibody - #DF13062
| Product: | HMBOX1 Antibody |
| Catalog: | DF13062 |
| Description: | Rabbit polyclonal antibody to HMBOX1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 47 kDa; 47kD(Calculated). |
| Uniprot: | Q6NT76 |
| RRID: | AB_2846023 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13062, RRID:AB_2846023.
Fold/Unfold
FLJ21616; HMBOX1; HMBOX1A; HMBX1_HUMAN; HNF1LA; homeobox containing 1; Homeobox containing protein 1; Homeobox-containing protein 1; homeobox-containing protein PBHNF; PBHNF;
Immunogens
Ubiquitous. Detected in pancreas, brain, spleen, placenta, prostate, thymus, liver, heart, bone marrow, skeletal muscle, stomach, uterus, testis, kidney, ovary, colon, lung, cardiac muscle and thyroid gland.
- Q6NT76 HMBX1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSSFPVVLLETMSHYTDEPRFTIEQIDLLQRLRRTGMTKHEILHALETLDRLDQEHSDKFGRRSSYGGSSYGNSTNNVPASSSTATASTQTQHSGMSPSPSNSYDTSPQPCTTNQNGRENNERLSTSNGKMSPTRYHANSMGQRSYSFEASEEDLDVDDKVEELMRRDSSVIKEEIKAFLANRRISQAVVAQVTGISQSRISHWLLQQGSDLSEQKKRAFYRWYQLEKTNPGATLSMRPAPIPIEDPEWRQTPPPVSATSGTFRLRRGSRFTWRKECLAVMESYFNENQYPDEAKREEIANACNAVIQKPGKKLSDLERVTSLKVYNWFANRRKEIKRRANIEAAILESHGIDVQSPGGHSNSDDVDGNDYSEQDDSTSHSDHQDPISLAVEMAAVNHTILALARQGANEIKTEALDDD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6NT76 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S126 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| K413 | Sumoylation | Uniprot |
Research Backgrounds
Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA. Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation. Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres (By similarity). Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination. Might also be involved in the DNA damage response at telomeres.
Nucleus. Cytoplasm. Chromosome>Telomere. Nucleus>Cajal body. Nucleus>PML body.
Note: Predominantly detected in cytoplasm (PubMed:16825764, PubMed:19728927). Localizes in a dynamic manner to actively processed telomeres (PubMed:23685356). Localizes to the periphery of Cajal bodies (PubMed:23685356). Associates with PML nuclear bodies in telomerase-negative cells (PubMed:23813958).
Nucleus. Cytoplasm.
Ubiquitous. Detected in pancreas, brain, spleen, placenta, prostate, thymus, liver, heart, bone marrow, skeletal muscle, stomach, uterus, testis, kidney, ovary, colon, lung, cardiac muscle and thyroid gland.
Associates with the telomerase holoenzyme complex. Interacts with DKC1, XRCC6 and COIL.
The homeobox domain is required for binding to 5'-TTAGGG-3' repeats in telomeres, and for telomere localization.
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