Product: HNRNPH3 Antibody
Catalog: DF13064
Description: Rabbit polyclonal antibody to HNRNPH3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 37kD(Calculated).
Uniprot: P31942
RRID: AB_2846025

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
HNRNPH3 Antibody detects endogenous levels of total HNRNPH3.
RRID:
AB_2846025
Cite Format: Affinity Biosciences Cat# DF13064, RRID:AB_2846025.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2H9; FLJ34092; Heterogeneous nuclear ribonucleoprotein H3; hnRNP 2H9; hnRNP H3; HNRNPH 3; HNRNPH3; HNRPH 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDWVMKHNGPNDASDGTVRLRGLPFGCSKEEIVQFFQGLEIVPNGITLTMDYQGRSTGEAFVQFASKEIAENALGKHKERIGHRYIEIFRSSRSEIKGFYDPPRRLLGQRPGPYDRPIGGRGGYYGAGRGSMYDRMRRGGDGYDGGYGGFDDYGGYNNYGYGNDGFDDRMRDGRGMGGHGYGGAGDASSGFHGGHFVHMRGLPFRATENDIANFFSPLNPIRVHIDIGADGRATGEADVEFVTHEDAVAAMSKDKNNMQHRYIELFLNSTPGGGSGMGGSGMGGYGRDGMDNQGGYGSVGRMGMGNNYSGGYGTPDGLGGYGRGGGGSGGYYGQGGMSGGGWRGMY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31942 As Substrate

Site PTM Type Enzyme
K6 Acetylation
K6 Methylation
K6 Sumoylation
K6 Ubiquitination
T17 Phosphorylation
R19 Methylation
S28 Phosphorylation
T47 Phosphorylation
T49 Phosphorylation
Y52 Phosphorylation
T57 Phosphorylation
S66 Phosphorylation
K67 Ubiquitination
K76 Ubiquitination
K78 Ubiquitination
R90 Methylation
K97 Ubiquitination
Y100 Phosphorylation
R110 Methylation
R116 Methylation
R121 Methylation
Y125 Phosphorylation
R129 Methylation
Y133 Phosphorylation
R135 Methylation
R138 Methylation
Y143 Phosphorylation
Y147 Phosphorylation
Y153 Phosphorylation
Y156 Phosphorylation
Y159 Phosphorylation
Y161 Phosphorylation
R174 Methylation
Y181 Phosphorylation
R200 Methylation
T207 Phosphorylation
S216 Phosphorylation
T270 Phosphorylation
S275 Phosphorylation
S280 Phosphorylation
Y296 Phosphorylation
S298 Phosphorylation
R301 Methylation
Y308 Phosphorylation
Y312 Phosphorylation
T314 Phosphorylation
Y321 Phosphorylation
R323 Methylation
S328 Phosphorylation
Y331 Phosphorylation
Y332 Phosphorylation
S338 Phosphorylation
R343 Methylation

Research Backgrounds

Function:

Involved in the splicing process and participates in early heat shock-induced splicing arrest. Due to their great structural variations the different isoforms may possess different functions in the splicing reaction.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.