HP1BP3 Antibody - #DF13070

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Product: HP1BP3 Antibody
Catalog: DF13070
Description: Rabbit polyclonal antibody to HP1BP3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 70 kDa; 61kD(Calculated).
Uniprot: Q5SSJ5
RRID: AB_2846031

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
HP1BP3 Antibody detects endogenous levels of total HP1BP3.
RRID:
AB_2846031
Cite Format: Affinity Biosciences Cat# DF13070, RRID:AB_2846031.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Heterochromatin protein 1, binding protein 3; Heterochromatin protein 1-binding protein 3; HP1 BP74; HP1B3_HUMAN; HP1BP 3; HP1BP3; MGC28927; MGC43701; MGC72624; OTTHUMP00000002717; OTTHUMP00000002719; OTTHUMP00000002720; Protein HP1 BP74; Protein HP1-BP74; RP5 930J4.3;

Immunogens

Immunogen:
Uniprot:

Q5SSJ5(HP1B3_HUMAN) >>Visit HPA database.

Gene(ID):
HP1BP3(50809)
Sequence:
MATDTSQGELVHPKALPLIVGAQLIHADKLGEKVEDSTMPIRRTVNSTRETPPKSKLAEGEEEKPEPDISSEESVSTVEEQENETPPATSSEAEQPKGEPENEEKEENKSSEETKKDEKDQSKEKEKKVKKTIPSWATLSASQLARAQKQTPMASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPKRRLQKKTPAKSPGKAASVKQRGSKPAPKVSAAQRGKARPLPKKAPPKAKTPAKKTRPSSTVIKKPSGGSSKKPATSARKEVKLPGKGKSTMKKSFRVKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
67
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5SSJ5 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
S6 Phosphorylation
K14 Ubiquitination
K29 Acetylation
T44 Phosphorylation
S47 Phosphorylation
T48 Phosphorylation
T51 Phosphorylation
S70 Phosphorylation
S71 Phosphorylation
S74 Phosphorylation
S76 Phosphorylation
T77 Phosphorylation
T85 Phosphorylation
S91 Phosphorylation
K131 Ubiquitination
S140 Phosphorylation
S142 Phosphorylation
R146 Methylation
K149 Ubiquitination
S155 Phosphorylation
S156 Phosphorylation
K160 Ubiquitination
K170 Ubiquitination
K175 Ubiquitination
S176 Phosphorylation
K190 Acetylation
K190 Ubiquitination
K204 Ubiquitination
K233 Ubiquitination
K240 Acetylation
K245 Acetylation
S248 Phosphorylation
S249 Phosphorylation
K258 Ubiquitination
K274 Ubiquitination
S277 Phosphorylation
Y278 Phosphorylation
S279 Phosphorylation
K283 Ubiquitination
Y284 Phosphorylation
S286 Phosphorylation
Y288 Phosphorylation
K291 Ubiquitination
K302 Ubiquitination
K320 Ubiquitination
K329 Ubiquitination
K366 Ubiquitination
T375 Phosphorylation
S377 Phosphorylation
Y379 Phosphorylation
K385 Ubiquitination
K393 Ubiquitination
K403 Ubiquitination
S418 Phosphorylation
S431 Phosphorylation
S441 Phosphorylation
S442 Phosphorylation
S446 Phosphorylation
T461 Phosphorylation
S465 Phosphorylation
K482 Ubiquitination
K508 Ubiquitination
S512 Phosphorylation
T514 Phosphorylation
S520 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation
T529 Phosphorylation
S530 Phosphorylation
T544 Phosphorylation
S548 Phosphorylation

Research Backgrounds

Function:

Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity. Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal.

Subcellular Location:

Nucleus. Chromosome.
Note: localized in nuclei but not in nucleoli in interphase. Colocalized with chromosomes in mitosis, with a gradually increased during G1 progression and a maximum level during late G1 phase (G1/S).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via PxVxL motif) with CBX5 (via Trp-174).

Family&Domains:

A central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA.

Restrictive clause

 

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