IFT122 Antibody - #DF13082

(3)  
Product: IFT122 Antibody
Catalog: DF13082
Description: Rabbit polyclonal antibody to IFT122
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 142 kDa; 142kD(Calculated).
Uniprot: Q9HBG6
RRID: AB_2846042

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
IFT122 Antibody detects endogenous levels of total IFT122.
RRID:
AB_2846042
Cite Format: Affinity Biosciences Cat# DF13082, RRID:AB_2846042.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CED; IF122_HUMAN; IFT122; Intraflagellar transport 122 homolog (Chlamydomonas); Intraflagellar transport protein 122 homolog; SPG; WD repeat domain 10; WD repeat-containing protein 10; WD repeat-containing protein 140; WDR10; WDR10p; WDR140;

Immunogens

Immunogen:
Uniprot:

Q9HBG6(IF122_HUMAN) >>Visit HPA database.

Gene(ID):
IFT122(55764)
Expression:
Q9HBG6 IF122_HUMAN:

Expressed in many tissues. Predominant expression in testis and pituitary.

Sequence:
MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFASGSADKSVIIWTSKLEGILKYTHNDAIQCVSYNPITHQLASCSSSDFGLWSPEQKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSSRWESFWMNRENEDAEDVIVNRYIQEIPSTLKSAVYSSQGSEAEEEEPEEEDDSPRDDNLEERNDILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRKKLAVVDENDTCLVYDIDTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHRQKLQGFVVGYNGSKIFCLHVFSISAVEVPQSAPMYQYLDRKLFKEAYQIACLGVTDTDWRELAMEALEGLDFETAKKAFIRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALEMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNIKEPKAAVEMYISAGEHVKAIEICGDHGWVDMLIDIARKLDKAEREPLLLCATYLKKLDSPGYAAETYLKMGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYMPYAQWLAENDRFEEAQKAFHKAGRQREAVQVLEQLTNNAVAESRFNDAAYYYWMLSMQCLDIAQDPAQKDTMLGKFYHFQRLAELYHGYHAIHRHTEDPFSVHRPETLFNISRFLLHSLPKDTPSGISKVKILFTLAKQSKALGAYRLARHAYDKLRGLYIPARFQKSIELGTLTIRAKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYDVLHLVEFYLEEGITDEEAISLIDLEVLRPKRDDRQLEIANNSSQILRLVETKDSIGDEDPFTAKLSFEQGGSEFVPVVVSRLVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHGCCPYCRRCKDDPGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
91
Bovine
91
Sheep
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBG6 As Substrate

Site PTM Type Enzyme
K10 Ubiquitination
K21 Ubiquitination
K50 Ubiquitination
K53 Ubiquitination
K66 Ubiquitination
K75 Ubiquitination
K83 Ubiquitination
K124 Ubiquitination
S131 Phosphorylation
S187 Phosphorylation
S192 Phosphorylation
K262 Ubiquitination
K271 Ubiquitination
K291 Ubiquitination
Y294 Phosphorylation
K302 Ubiquitination
K309 Ubiquitination
K382 Ubiquitination
Y412 Phosphorylation
Y415 Phosphorylation
Y424 Phosphorylation
K463 Ubiquitination
K477 Ubiquitination
S523 Phosphorylation
S525 Phosphorylation
K528 Ubiquitination
Y624 Phosphorylation
K631 Ubiquitination
K663 Ubiquitination
Y674 Phosphorylation
S679 Phosphorylation
K686 Ubiquitination
K705 Ubiquitination
K711 Ubiquitination
K745 Ubiquitination
K748 Ubiquitination
K753 Ubiquitination
K762 Ubiquitination
K765 Ubiquitination
K816 Ubiquitination
K817 Ubiquitination
K830 Ubiquitination
K835 Ubiquitination
K856 Ubiquitination
K861 Ubiquitination
K883 Ubiquitination
K987 Ubiquitination
K995 Ubiquitination
K997 Ubiquitination
T1001 Phosphorylation
K1004 Ubiquitination
S1006 Phosphorylation
K1007 Ubiquitination
K1021 Acetylation
K1021 Ubiquitination
K1033 Ubiquitination
K1045 Ubiquitination
K1141 Ubiquitination
S1143 Phosphorylation
K1153 Ubiquitination
K1184 Ubiquitination

Research Backgrounds

Function:

As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is required in ciliogenesis and ciliary protein trafficking. Involved in cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity).

Subcellular Location:

Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to photoreceptor connecting cilia.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues. Predominant expression in testis and pituitary.

Subunit Structure:

Component of the IFT complex A (IFT-A) complex. IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts with IFT43:WDR35; the interaction connects the 2 IFT-A subcomplexes. Interacts with C11orf74; the interaction associates C11orf74 with IFT-A complex.

Family&Domains:

Forms the trimeric core subcomplex IFT122:IFT140:WDR19 via the C-terminal region, whereas it interacts with IFT43:WDR35 via the N-terminal region containing the WD repeats.

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