IIP45 Antibody - #DF13086

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Product: IIP45 Antibody
Catalog: DF13086
Description: Rabbit polyclonal antibody to IIP45
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 45 kDa; 43kD(Calculated).
Uniprot: Q5JXC2
RRID: AB_2846046

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
IIP45 Antibody detects endogenous levels of total IIP45.
RRID:
AB_2846046
Cite Format: Affinity Biosciences Cat# DF13086, RRID:AB_2846046.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ12438; FLJ38609; IGFBP 2 binding protein; IGFBP2 binding protein; IIP45; Invasion inhibitory protein 45; Migration and invasion inhibitory protein; RP5 1077B9.4;

Immunogens

Immunogen:
Uniprot:

Q5JXC2(MIIP_HUMAN) >>Visit HPA database.

Gene(ID):
MIIP(60672)
Expression:
Q5JXC2 MIIP_HUMAN:

Ubiquitous. Isoform 1 is expressed in brain but underexpressed in glioma tissues, at protein level. Isoform 2 is not detected in normal organs, but is expressed in gliomas with increasing levels with glioma progression. On the contrary, at protein level, isoform 2 is not detected in gliomas, suggesting that this isoform is unstable in glioma cells.

Sequence:
MVEAEELAQLRLLNLELLRQLWVGQDAVRRSVARAASESSLESSSSYNSETPSTPETSSTSLSTSCPRGRSSVWGPPDACRGDLRDVARSGVASLPPAKCQHQESLGRPRPHSAPSLGTSSLRDPEPSGRLGDPGPQEAQTPRSILAQQSKLSKPRVTFSEESAVPKRSWRLRPYLGYDWIAGSLDTSSSITSQPEAFFSKLQEFRETNKEECICSHPEPQLPGLRESSGSGVEEDHECVYCYRVNRRLFPVPVDPGTPCRLCRTPRDQQGPGTLAQPAHVRVSIPLSILEPPHRYHIHRRKSFDASDTLALPRHCLLGWDIFPPKSEKSSAPRNLDLWSSVSAEAQHQKLSGTSSPFHPASPMQMLPPTPTWSVPQVPRPHVPRQKP

PTMs - Q5JXC2 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
S45 Phosphorylation
S49 Phosphorylation
T54 Phosphorylation
T57 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
K99 Ubiquitination
S113 Phosphorylation
T141 Phosphorylation
K210 Ubiquitination
Y241 Phosphorylation
T258 Phosphorylation
S303 Phosphorylation
S352 Phosphorylation
T354 Phosphorylation
S362 Phosphorylation
T370 Phosphorylation
T372 Phosphorylation

Research Backgrounds

Function:

Inhibits glioma cells invasion and down-regulates adhesion- and motility-associated genes such as NFKB2 and ICAM1. Exhibits opposing effects to IGFBP2 on cell invasion.

PTMs:

Isoform 2 is degraded by the ubiquitin-proteasome pathway.

Tissue Specificity:

Ubiquitous. Isoform 1 is expressed in brain but underexpressed in glioma tissues, at protein level. Isoform 2 is not detected in normal organs, but is expressed in gliomas with increasing levels with glioma progression. On the contrary, at protein level, isoform 2 is not detected in gliomas, suggesting that this isoform is unstable in glioma cells.

Subunit Structure:

Interacts with IGFBP2.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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