INTS12 Antibody | Affinity Biosciences

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INTS12 Antibody - Western blot analysis of extracts from HepG2 cells, using INTS12 Antibody.

Product:	INTS12 Antibody
Catalog:	DF13091
Description:	Rabbit polyclonal antibody to INTS12
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49 kDa; 49kD(Calculated).
Uniprot:	Q96CB8
RRID:	AB_2846051

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%)

Clonality:

Polyclonal

Specificity:

INTS12 Antibody detects endogenous levels of total INTS12.

RRID:

AB_2846051
Cite Format: Affinity Biosciences Cat# DF13091, RRID:AB_2846051.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Hypothetical Nuclear Factor SBBI22; INT12; Integrator complex subunit 12; PHD finger protein 22; SBBI22;

Immunogens

Immunogen:

Uniprot:

Q96CB8(INT12_HUMAN) >>Visit HPA database.

Gene(ID):

INTS12(57117)

Sequence:

Q96CB8 INT12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAATVNLELDPIFLKALGFLHSKSKDSAEKLKALLDESLARGIDSSYRPSQKDVEPPKISSTKNISIKQEPKISSSLPSGNNNGKVLTTEKVKKEAEKRPADKMKSDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKTTSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

100

Horse

Bovine

Sheep

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96CB8 As Substrate

Q96CB8

Site	PTM Type	Source
S66	Phosphorylation	Uniprot
K68	Acetylation	Uniprot
K68	Sumoylation	Uniprot
K85	Acetylation	Uniprot
K91	Acetylation	Uniprot
K94	Acetylation	Uniprot
K105	Sumoylation	Uniprot
K122	Methylation	Uniprot
T125	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
S128	Phosphorylation	Uniprot
K192	Ubiquitination	Uniprot
K198	Ubiquitination	Uniprot
K222	Acetylation	Uniprot
K229	Acetylation	Uniprot
K247	Ubiquitination	Uniprot
K254	Sumoylation	Uniprot
K263	Acetylation	Uniprot
K320	Ubiquitination	Uniprot
K329	Acetylation	Uniprot
K329	Ubiquitination	Uniprot
T355	Phosphorylation	Uniprot
K361	Acetylation	Uniprot
T371	Phosphorylation	Uniprot
S378	Phosphorylation	Uniprot
S383	Phosphorylation	Uniprot
S389	Phosphorylation	Uniprot
K419	Methylation	Uniprot
S427	Phosphorylation	Uniprot
K433	Methylation	Uniprot
S437	Phosphorylation	Uniprot
K446	Acetylation	Uniprot
K446	Ubiquitination	Uniprot

Research Backgrounds

Q96CB8_INT12_HUMAN

Function:

Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.

Subcellular Location:

Nucleus.

Subunit Structure:

Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L and INTS12.

Family&Domains:

Belongs to the Integrator subunit 12 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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INTS12 Antibody - #DF13091