LAS1L Antibody | Affinity Biosciences

Product:	LAS1L Antibody
Catalog:	DF13123
Description:	Rabbit polyclonal antibody to LAS1L
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse
Mol.Wt.:	83 kDa; 83kD(Calculated).
Uniprot:	Q9Y4W2
RRID:	AB_2846083

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%)

Clonality:

Polyclonal

Specificity:

LAS1L Antibody detects endogenous levels of total LAS1L.

RRID:

AB_2846083
Cite Format: Affinity Biosciences Cat# DF13123, RRID:AB_2846083.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dJ475B7.2; FLJ12525; FLJ30133; FLJ45274; LAS1 like (S. cerevisiae); Las1 like; LAS1L; LAS1L_HUMAN; Protein LAS1 homolog; Ribosomal biogenesis protein LAS1L; RP3 475B7.2;

Immunogens

Immunogen:

Uniprot:

Q9Y4W2(LAS1L_HUMAN) >>Visit HPA database.

Gene(ID):

LAS1L(81887)

Sequence:

Q9Y4W2 LAS1L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSWESGAGPGLGSQGMDLVWSAWYGKCVKGKGSLPLSAHGIVVAWLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLDVTGGLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCLAQEVNIPDWIVDLRHELTHKKMPHINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEFREGIEEEDQEEDKNIVVDDITEQKPEPQDDGKSTESDVKADGDSKGSEEVDSHCKKALSHKELYERARELLVSYEEEQFTVLEKFRYLPKAIKAWNNPSPRVECVLAELKGVTCENREAVLDAFLDDGFLVPTFEQLAALQIEYEDGQTEVQRGEGTDPKSHKNVDLNDVLVPKPFSQFWQPLLRGLHSQNFTQALLERMLSELPALGISGIRPTYILRWTVELIVANTKTGRNARRFSAGQWEARRGWRLFNCSASLDWPRMVESCLGSPCWASPQLLRIIFKAMGQGLPDEEQEKLLRICSIYTQSGENSLVQEGSEASPIGKSPYTLDSLYWSVKPASSSFGSEAKAQQQEEQGSVNDVKEEEKEEKEVLPDQVEEEEENDDQEEEEEDEDDEDDEEEDRMEVGPFSTGQESPTAENARLLAQKRGALQGSAWQVSSEDVRWDTFPLGRMPGQTEDPAELMLENYDTMYLLDQPVLEQRLEPSTCKTDTLGLSCGVGSGNCSNSSSSNFEGLLWSQGQLHGLKTGLQLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4W2 As Substrate

Q9Y4W2

Site	PTM Type	Source
S21	Phosphorylation	Uniprot
Y24	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
K64	Ubiquitination	Uniprot
S90	Phosphorylation	Uniprot
T91	Phosphorylation	Uniprot
K98	Ubiquitination	Uniprot
Y115	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
K139	Methylation	Uniprot
K139	Ubiquitination	Uniprot
K161	Ubiquitination	Uniprot
K162	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
K226	Sumoylation	Uniprot
K226	Ubiquitination	Uniprot
K234	Ubiquitination	Uniprot
S235	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
K241	Ubiquitination	Uniprot
S246	Phosphorylation	Uniprot
S249	Phosphorylation	Uniprot
K257	Ubiquitination	Uniprot
S261	Phosphorylation	Uniprot
K263	Ubiquitination	Uniprot
K286	Ubiquitination	Uniprot
K292	Ubiquitination	Uniprot
K295	Sumoylation	Uniprot
K295	Ubiquitination	Uniprot
S301	Phosphorylation	Uniprot
K312	Ubiquitination	Uniprot
K362	Ubiquitination	Uniprot
S363	Phosphorylation	Uniprot
K365	Ubiquitination	Uniprot
K376	Ubiquitination	Uniprot
K432	Ubiquitination	Uniprot
S441	Phosphorylation	Uniprot
S477	Phosphorylation	Uniprot
K486	Ubiquitination	Uniprot
K499	Ubiquitination	Uniprot
S510	Phosphorylation	Uniprot
S514	Phosphorylation	Uniprot
S523	Phosphorylation	Uniprot
S528	Phosphorylation	Uniprot
S534	Phosphorylation	Uniprot
Y536	Phosphorylation	Uniprot
S538	Phosphorylation	Uniprot
K540	Ubiquitination	Uniprot
S543	Phosphorylation	Uniprot
S545	Phosphorylation	Uniprot
S548	Phosphorylation	Uniprot
K551	Ubiquitination	Uniprot
S560	Phosphorylation	Uniprot
K565	Sumoylation	Uniprot
K565	Ubiquitination	Uniprot
S612	Phosphorylation	Uniprot
T613	Phosphorylation	Uniprot
S617	Phosphorylation	Uniprot
T619	Phosphorylation	Uniprot
K691	Ubiquitination	Uniprot

Research Backgrounds

Q9Y4W2_LAS1L_HUMAN

Function:

Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.

Subcellular Location:

Nucleus>Nucleolus. Nucleus>Nucleoplasm. Cytoplasm.
Note: Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Localizes mainly to the granular component, the region implicated in the later steps of rRNA processing and subunit assembly and export.

Subunit Structure:

Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148.

Family&Domains:

Belongs to the LAS1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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LAS1L Antibody - #DF13123