MAP1S Antibody - #DF13137

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Product: MAP1S Antibody
Catalog: DF13137
Description: Rabbit polyclonal antibody to MAP1S
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 130 kDa; 112kD(Calculated).
Uniprot: Q66K74
RRID: AB_2846097

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
MAP1S Antibody detects endogenous levels of total MAP1S.
RRID:
AB_2846097
Cite Format: Affinity Biosciences Cat# DF13137, RRID:AB_2846097.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BPY2-interacting protein 1; BPY2IP1; C19orf5; FLJ10669; MAP-1S; MAP1S; MAP1S light chain; MAP1S_HUMAN; MAP8; MGC133087; microtubule-associated protein 1S; Microtubule-associated protein 8; Variable charge Y chromosome 2-interacting protein 1; VCY2-interacting protein 1; VCY2IP-1; VCY2IP1;

Immunogens

Immunogen:
Uniprot:

Q66K74(MAP1S_HUMAN) >>Visit HPA database.

Gene(ID):
MAP1S(55201)
Expression:
Q66K74 MAP1S_HUMAN:

Expressed in neurons (at protein level). Expressed in spermatocytes, spermatids and spermatozoa. Expressed in the cerebral cortex. Highly expressed in testis. Moderately expressed in the brain, colon, heart, kidney, liver, lung, placenta, small intestine, spleen and stomach. Weakly expressed in muscle.

Sequence:
MAAVAGSGAAAAPSSLLLVVGSEFGSPGLLTYVLEELERGIRSWDVDPGVCNLDEQLKVFVSRHSATFSSIVKGQRSLHHRGDNLETLVLLNPSDKSLYDELRNLLLDPASHKLLVLAGPCLEETGELLLQTGGFSPHHFLQVLKDREIRDILATTPPPVQPPILTITCPTFGDWAQLAPAVPGLQGALRLQLRLNPPAQLPNSEGLCEFLEYVAESLEPPSPFELLEPPTSGGFLRLGRPCCYIFPGGLGDAAFFAVNGFTVLVNGGSNPKSSFWKLVRHLDRVDAVLVTHPGADSLPGLNSLLRRKLAERSEVAAGGGSWDDRLRRLISPNLGVVFFNACEAASRLARGEDEAELALSLLAQLGITPLPLSRGPVPAKPTVLFEKMGVGRLDMYVLHPPSAGAERTLASVCALLVWHPAGPGEKVVRVLFPGCTPPACLLDGLVRLQHLRFLREPVVTPQDLEGPGRAESKESVGSRDSSKREGLLATHPRPGQERPGVARKEPARAEAPRKTEKEAKTPRELKKDPKPSVSRTQPREVRRAASSVPNLKKTNAQAAPKPRKAPSTSHSGFPPVANGPRSPPSLRCGEASPPSAACGSPASQLVATPSLELGPIPAGEEKALELPLAASSIPRPRTPSPESHRSPAEGSERLSLSPLRGGEAGPDASPTVTTPTVTTPSLPAEVGSPHSTEVDESLSVSFEQVLPPSAPTSEAGLSLPLRGPRARRSASPHDVDLCLVSPCEFEHRKAVPMAPAPASPGSSNDSSARSQERAGGLGAEETPPTSVSESLPTLSDSDPVPLAPGAADSDEDTEGFGVPRHDPLPDPLKVPPPLPDPSSICMVDPEMLPPKTARQTENVSRTRKPLARPNSRAAAPKATPVAAAKTKGLAGGDRASRPLSARSEPSEKGGRAPLSRKSSTPKTATRGPSGSASSRPGVSATPPKSPVYLDLAYLPSGSSAHLVDEEFFQRVRALCYVISGQDQRKEEGMRAVLDALLASKQHWDRDLQVTLIPTFDSVAMHTWYAETHARHQALGITVLGSNSMVSMQDDAFPACKVEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
80
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q66K74 As Substrate

Site PTM Type Enzyme
K73 Acetylation
K73 Ubiquitination
K96 Ubiquitination
S111 Phosphorylation
K277 Ubiquitination
S313 Phosphorylation
S321 Phosphorylation
K380 Ubiquitination
K387 Ubiquitination
S472 Phosphorylation
S475 Phosphorylation
S478 Phosphorylation
T490 Phosphorylation
T521 Phosphorylation
S546 Phosphorylation
S547 Phosphorylation
K552 Methylation
K553 Methylation
T554 Phosphorylation
S582 Phosphorylation
S592 Phosphorylation
S595 Phosphorylation
S603 Phosphorylation
S610 Phosphorylation
S631 Phosphorylation
S632 Phosphorylation
T638 Phosphorylation
S640 Phosphorylation
S643 Phosphorylation
S646 Phosphorylation
S655 Phosphorylation
S657 Phosphorylation
T671 Phosphorylation
T673 Phosphorylation
T676 Phosphorylation
S729 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
S759 Phosphorylation
S762 Phosphorylation
S763 Phosphorylation
S766 Phosphorylation
S767 Phosphorylation
S770 Phosphorylation
T782 Phosphorylation
S788 Phosphorylation
S790 Phosphorylation
S797 Phosphorylation
S809 Phosphorylation
T813 Phosphorylation
T852 Phosphorylation
R854 Methylation
R861 Methylation
R863 Methylation
S871 Phosphorylation
K877 Ubiquitination
T879 Phosphorylation
T886 Phosphorylation
K887 Methylation
R894 Methylation
S896 Phosphorylation
S900 Phosphorylation
S903 Phosphorylation
S906 Phosphorylation
R926 Methylation
S929 Phosphorylation
R935 Methylation
T941 Phosphorylation
S999 Phosphorylation
K1000 Ubiquitination

Research Backgrounds

Function:

Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles (By similarity).

Subcellular Location:

Nucleus. Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.
Note: Detected in filopodia-like protrusions and synapses (By similarity). Detected in perinuclear punctate network corresponding to mitochondrial aggregates and in the nucleus in cells exhibiting apoptosis. Associated specifically with microtubules stabilized by paclitaxel and colocalizes with RASSF1 isoform A. In interphase cells, shows a diffuse cytoplasmic staining with partial localization to the microtubules. During the different stages of mitosis detected at the spindle microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in neurons (at protein level). Expressed in spermatocytes, spermatids and spermatozoa. Expressed in the cerebral cortex. Highly expressed in testis. Moderately expressed in the brain, colon, heart, kidney, liver, lung, placenta, small intestine, spleen and stomach. Weakly expressed in muscle.

Subunit Structure:

Heterodimer of a heavy and a light chain. Interacts with microtubules and actin. Both MAP1S heavy and light chains interact with microtubules. MAP1S light chain interacts with actin. Interacts (via C-terminus) with GAN (via Kelch domains) (By similarity). Interacts with ESR1, LRPPRC, RASSF1 isoform A and isoform C, microtubules and VCY2. Interacts with WDR47 (via N-terminus of light chain).

Family&Domains:

The N-terminus of the heavy chain associates with the C-terminus of the light chain to form the heterodimer complex (By similarity). Its C-terminal part of the heavy chain interacts with ESR1.

Belongs to the MAP1 family.

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