METTL1 Antibody - #DF13143
| Product: | METTL1 Antibody |
| Catalog: | DF13143 |
| Description: | Rabbit polyclonal antibody to METTL1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 31 kDa; 31kD(Calculated). |
| Uniprot: | Q9UBP6 |
| RRID: | AB_2846103 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13143, RRID:AB_2846103.
Fold/Unfold
C12orf1; D1075 like gene product; FLJ95748; Methyltransferase like 1; Methyltransferase like protein 1; Methyltransferase-like protein 1; Mettl1; TRM8; TRMB_HUMAN; TRMT8; tRNA (guanine N(7) ) methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46) methyltransferase; tRNA(m7G46)-methyltransferase; YDL201w;
Immunogens
- Q9UBP6 TRMB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAETRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEFADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGGFQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSLPGH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBP6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| R24 | Methylation | Uniprot | |
| S27 | Phosphorylation | Q15418 (RPS6KA1) , P31749 (AKT1) | Uniprot |
| K111 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot |
Research Backgrounds
Methyltransferase that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs regulates translation. Also methylates a specific subset of miRNAs, such as let-7. N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA). Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).
Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.
Nucleus.
Ubiquitous.
Forms a complex with WDR4.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
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For Research Use Only.
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