MTF2 Antibody | Affinity Biosciences

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MTF2 Antibody - Western blot analysis of extracts from HepG2 cells, using MTF2 Antibody.

Product:	MTF2 Antibody
Catalog:	DF13158
Description:	Rabbit polyclonal antibody to MTF2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	60 kDa; 67kD(Calculated).
Uniprot:	Q9Y483
RRID:	AB_2846118

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

MTF2 Antibody detects endogenous levels of total MTF2.

RRID:

AB_2846118
Cite Format: Affinity Biosciences Cat# DF13158, RRID:AB_2846118.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DJ976O13.2; hPCl2; M96; metal regulatory transcription factor 2; Metal response element binding transcription factor 2; metal-response element DNA-binding protein M96; PCL2; polycomb-like 2; polycomb-like protein 2; Putative DNA binding protein; RP5 976O13.1; RP5-976O13.1; TDRD19A; tudor domain containing 19A;

Immunogens

Immunogen:

Uniprot:

Q9Y483(MTF2_HUMAN) >>Visit HPA database.

Gene(ID):

MTF2(22823)

Sequence:

Q9Y483 MTF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKINILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDSSVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPGDWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVIHKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRVPPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEPLLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWPAAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAGRIACGEKYRVLARRVTLDGKVQYLVEWEGATAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y483 As Substrate

Q9Y483

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
T24	Phosphorylation	Uniprot
S27	Phosphorylation	Uniprot
T29	Phosphorylation	Uniprot
K30	Methylation	Uniprot
K45	Methylation	Uniprot
K161	Ubiquitination	Uniprot
K179	Ubiquitination	Uniprot
Y245	Phosphorylation	Uniprot
K313	Ubiquitination	Uniprot
Y327	Phosphorylation	Uniprot
K378	Methylation	Uniprot
S401	Phosphorylation	Uniprot
K412	Methylation	Uniprot
K416	Methylation	Uniprot
K424	Ubiquitination	Uniprot
S438	Phosphorylation	Uniprot
S452	Phosphorylation	Uniprot
K463	Ubiquitination	Uniprot
Y474	Phosphorylation	Uniprot
S479	Phosphorylation	Uniprot
R480	Methylation	Uniprot
S488	Phosphorylation	Uniprot
K522	Ubiquitination	Uniprot

Research Backgrounds

Q9Y483_MTF2_HUMAN

Function:

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Associated component of the PRC2 complex.

Family&Domains:

The Tudor domain recognizes and binds H3K36me3 (PubMed:23142980, PubMed:23228662).

Belongs to the Polycomblike family.

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MTF2 Antibody - #DF13158