MYO1D Antibody - #DF13166

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Product: MYO1D Antibody
Catalog: DF13166
Description: Rabbit polyclonal antibody to MYO1D
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 100 kDa; 116kD(Calculated).
Uniprot: O94832
RRID: AB_2846126

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(82%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
MYO1D Antibody detects endogenous levels of total MYO1D.
RRID:
AB_2846126
Cite Format: Affinity Biosciences Cat# DF13166, RRID:AB_2846126.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0727; Myosin I gamma; Myosin Id; MYR4;

Immunogens

Immunogen:
Uniprot:

O94832(MYO1D_HUMAN) >>Visit HPA database.

Gene(ID):
MYO1D(4642)
Expression:
O94832 MYO1D_HUMAN:

Expressed in many tissues. Highest levels in brain, followed by lung and ovary; expression is lowest in spleen.

Sequence:
MAEQESLEFGKADFVLMDTVSMPEFMANLRLRFEKGRIYTFIGEVVVSVNPYKLLNIYGRDTIEQYKGRELYERPPHLFAIADAAYKAMKRRSKDTCIVISGESGAGKTEASKYIMQYIAAITNPSQRAEVERVKNMLLKSNCVLEAFGNAKTNRNDNSSRFGKYMDINFDFKGDPIGGHINNYLLEKSRVIVQQPGERSFHSFYQLLQGGSEQMLRSLHLQKSLSSYNYIHVGAQLKSSINDAAEFRVVADAMKVIGFKPEEIQTVYKILAAILHLGNLKFVVDGDTPLIENGKVVSIIAELLSTKTDMVEKALLYRTVATGRDIIDKQHTEQEASYGRDAFAKAIYERLFCWIVTRINDIIEVKNYDTTIHGKNTVIGVLDIYGFEIFDNNSFEQFCINYCNEKLQQLFIQLVLKQEQEEYQREGIPWKHIDYFNNQIIVDLVEQQHKGIIAILDDACMNVGKVTDEMFLEALNSKLGKHAHFSSRKLCASDKILEFDRDFRIRHYAGDVVYSVIGFIDKNKDTLFQDFKRLMYNSSNPVLKNMWPEGKLSITEVTKRPLTAATLFKNSMIALVDNLASKEPYYVRCIKPNDKKSPQIFDDERCRHQVEYLGLLENVRVRRAGFAFRQTYEKFLHRYKMISEFTWPNHDLPSDKEAVKKLIERCGFQDDVAYGKTKIFIRTPRTLFTLEELRAQMLIRIVLFLQKVWRGTLARMRYKRTKAALTIIRYYRRYKVKSYIHEVARRFHGVKTMRDYGKHVKWPSPPKVLRRFEEALQTIFNRWRASQLIKSIPASDLPQVRAKVAAVEMLKGQRADLGLQRAWEGNYLASKPDTPQTSGTFVPVANELKRKDKYMNVLFSCHVRKVNRFSKVEDRAIFVTDRHLYKMDPTKQYKVMKTIPLYNLTGLSVSNGKDQLVVFHTKDNKDLIVCLFSKQPTHESRIGELVGVLVNHFKSEKRHLQVNVTNPVQCSLHGKKCTVSVETRLNQPQPDFTKNRSGFILSVPGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
91
Rabbit
91
Horse
82
Xenopus
73
Chicken
73
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94832 As Substrate

Site PTM Type Enzyme
Y39 Phosphorylation
Y52 Phosphorylation
Y58 Phosphorylation
Y72 Phosphorylation
S93 Phosphorylation
K108 Ubiquitination
Y114 Phosphorylation
T153 Phosphorylation
K164 Ubiquitination
K188 Ubiquitination
S200 Phosphorylation
S203 Phosphorylation
Y205 Phosphorylation
S212 Phosphorylation
S224 Phosphorylation
S239 Phosphorylation
T308 Phosphorylation
Y317 Phosphorylation
K329 Ubiquitination
Y368 Phosphorylation
Y423 Phosphorylation
Y435 Phosphorylation
S493 Phosphorylation
R501 Methylation
Y514 Phosphorylation
K524 Ubiquitination
Y536 Phosphorylation
S553 Phosphorylation
K559 Ubiquitination
T563 Phosphorylation
Y585 Phosphorylation
Y586 Phosphorylation
Y612 Phosphorylation
Y674 Phosphorylation
T683 Phosphorylation
T726 Phosphorylation
Y734 Phosphorylation
S738 Phosphorylation
Y756 Phosphorylation
K767 Ubiquitination
K811 Methylation
K811 Ubiquitination
K831 Ubiquitination
T834 Phosphorylation
K886 Ubiquitination
T921 Phosphorylation
S955 Phosphorylation
S971 Phosphorylation
K976 Ubiquitination
S997 Phosphorylation

Research Backgrounds

Function:

Unconventional myosin that functions as actin-based motor protein with ATPase activity (By similarity). Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins from early to recycling endosomes (By similarity). Required for normal planar cell polarity in ciliated tracheal cells, for normal rotational polarity of cilia, and for coordinated, unidirectional ciliary movement in the trachea. Required for normal, polarized cilia organization in brain ependymal epithelial cells (By similarity).

Subcellular Location:

Cytoplasm. Perikaryon. Cell projection>Dendrite. Early endosome. Cytoplasm>Cell cortex.
Note: Colocalizes with the actin cytoskeleton in the cell cortex close to the apical cell membrane. Colocalizes with cytoplasmic puncta that are reminiscent of transport vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in many tissues. Highest levels in brain, followed by lung and ovary; expression is lowest in spleen.

Subunit Structure:

Interacts (via the two IQ motifs) with calmodulin. Binds an additional calmodulin chain via a third, C-terminal region. Interacts with F-actin.

Family&Domains:

Binds a calmodulin chain via each of the two IQ domains. IQ domain 1 mediates interaction with calmodulin both in the presence and in the absence of Ca(2+). IQ domain 2 mediates interaction with calmodulin in the presence of Ca(2+).

The TH1 domain is required for activity in complementing zebrafish defects in Kupffer's vesicle lumen size.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Restrictive clause

 

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