MYO1E Antibody - #DF13167

(3)  
Product: MYO1E Antibody
Catalog: DF13167
Description: Rabbit polyclonal antibody to MYO1E
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 127 kDa; 127kD(Calculated).
Uniprot: Q12965
RRID: AB_2846127

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Horse(92%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MYO1E Antibody detects endogenous levels of total MYO1E.
RRID:
AB_2846127
Cite Format: Affinity Biosciences Cat# DF13167, RRID:AB_2846127.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FSGS6; HuncM-IC; MYO1C; Myo1e; MYO1E variant protein; MYO1E_HUMAN; Myosin heavy chain myr 3; Myosin IC, formerly; Myosin IE; Myosin-Ic; Myr 3; Myr3; MYR5; Unconventional myosin 1E; Unconventional myosin Ie; Unconventional myosin-Ie;

Immunogens

Immunogen:
Uniprot:

Q12965(MYO1E_HUMAN) >>Visit HPA database.

Gene(ID):
MYO1E(4643)
Expression:
Q12965 MYO1E_HUMAN:

Expressed in the immune system. In the kidney, predominantly expressed in the glomerulus, including podocytes.

Sequence:
MGSKGVYQYHWQSHNVKHSGVDDMVLLSKITENSIVENLKKRYMDDYIFTYIGSVLISVNPFKQMPYFGEKEIEMYQGAAQYENPPHIYALADNMYRNMIIDRENQCVIISGESGAGKTVAAKYIMSYISRVSGGGTKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEGASAEQKHSLGITSMDYYYYLSLSGSYKVDDIDDRREFQETLHAMNVIGIFAEEQTLVLQIVAGILHLGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGGKSESIHVTLNVEQACYTRDALAKALHARVFDFLVDSINKAMEKDHEEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIEYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGSHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFMDLIELMQSSELPFIKSLFPENLQADKKGRPTTAGSKIKKQANDLVSTLMKCTPHYIRCIKPNETKKPRDWEESRVKHQVEYLGLKENIRVRRAGYAYRRIFQKFLQRYAILTKATWPSWQGEEKQGVLHLLQSVNMDSDQFQLGRSKVFIKAPESLFLLEEMRERKYDGYARVIQKSWRKFVARKKYVQMREEASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKREKIDFADTVTKYDRRFKGVKRDLLLTPKCLYLIGREKVKQGPDKGLVKEVLKRKIEIERILSVSLSTMQDDIFILHEQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGFGDLAVLKPSNKVLQVSIGPGLPKNSRPTRRNTTQNTGYSSGTQNANYPVRAAPPPPGYHQNGVIRNQYVPYPHAPGSQRSNQKSLYTSMARPPLPRQQSTSSDRVSQTPESLDFLKVPDQGAAGVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDIIDIIKEDPSGWWTGRLRGKQGLFPNNYVTKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Dog
92
Rabbit
83
Bovine
75
Sheep
75
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12965 As Substrate

Site PTM Type Enzyme
Y7 Phosphorylation
Y9 Phosphorylation
K17 Ubiquitination
K29 Ubiquitination
K40 Ubiquitination
Y47 Phosphorylation
Y51 Phosphorylation
Y67 Phosphorylation
R103 Methylation
K118 Ubiquitination
T137 Phosphorylation
K138 Ubiquitination
K143 Ubiquitination
K160 Ubiquitination
K188 Ubiquitination
K196 Ubiquitination
S197 Phosphorylation
K320 Ubiquitination
K322 Ubiquitination
K331 Ubiquitination
S370 Phosphorylation
Y440 Phosphorylation
K444 Ubiquitination
K453 Ubiquitination
K554 Ubiquitination
K555 Ubiquitination
T560 Phosphorylation
K564 Ubiquitination
K604 Ubiquitination
Y609 Phosphorylation
K613 Acetylation
K613 Ubiquitination
Y623 Phosphorylation
Y625 Phosphorylation
K631 Acetylation
K631 Ubiquitination
K641 Ubiquitination
K694 Ubiquitination
Y698 Phosphorylation
K704 Ubiquitination
Y715 Phosphorylation
K729 Ubiquitination
S736 Phosphorylation
K760 Ubiquitination
K763 Ubiquitination
K772 Ubiquitination
K805 Ubiquitination
K809 Ubiquitination
K859 Ubiquitination
K872 Ubiquitination
T876 Phosphorylation
K880 Ubiquitination
K883 Ubiquitination
S890 Phosphorylation
K910 Ubiquitination
K914 Ubiquitination
S919 Phosphorylation
S928 Phosphorylation
T935 Phosphorylation
T936 Phosphorylation
T939 Phosphorylation
Y941 Phosphorylation
S943 Phosphorylation
Y950 Phosphorylation
Y961 Phosphorylation
Y971 Phosphorylation
Y974 Phosphorylation
S980 Phosphorylation
S983 Phosphorylation
S987 Phosphorylation
Y989 Phosphorylation
T990 Phosphorylation
S991 Phosphorylation
S1002 Phosphorylation
T1003 Phosphorylation
S1004 Phosphorylation
S1005 Phosphorylation
T1011 Phosphorylation
K1019 Ubiquitination

Research Backgrounds

Function:

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle. Cytoplasmic vesicle>Clathrin-coated vesicle. Cell junction.
Note: Colocalizes with F-actin (By similarity). In cultured podocytes, it localizes close to and is associated with the cytoplasmic membrane, with enrichment at the lamellipodia tips. Colocalizes with cytoplasmic vesicles, including endocytic clathrin-coated vesicles. Colocalizes with dynamin at cytoplasmic vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the immune system. In the kidney, predominantly expressed in the glomerulus, including podocytes.

Subunit Structure:

Interacts with CALM and F-actin. Interacts (via SH3 domain) with SYNJ1, DNM1 and DNM2. Interacts with ARL14EP. Interacts with CARMIL1.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

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