Myoferlin Antibody - #DF13168

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Product: Myoferlin Antibody
Catalog: DF13168
Description: Rabbit polyclonal antibody to Myoferlin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 235 kDa; 235kD(Calculated).
Uniprot: Q9NZM1
RRID: AB_2846128

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(91%), Rabbit(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Myoferlin Antibody detects endogenous levels of total Myoferlin.
RRID:
AB_2846128
Cite Format: Affinity Biosciences Cat# DF13168, RRID:AB_2846128.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Fer 1 like 3, myoferlin (C. elegans); Fer 1 like family member 3; Fer 1 like protein 3; Fer-1-like protein 3; FER1L3; myoF; MYOF_HUMAN; Myoferlin;

Immunogens

Immunogen:
Uniprot:

Q9NZM1(MYOF_HUMAN) >>Visit HPA database.

Gene(ID):
MYOF(26509)
Expression:
Q9NZM1 MYOF_HUMAN:

Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.

Sequence:
MLRVIVESASNIPKTKFGKPDPIVSVIFKDEKKKTKKVDNELNPVWNEILEFDLRGIPLDFSSSLGIIVKDFETIGQNKLIGTATVALKDLTGDQSRSLPYKLISLLNEKGQDTGATIDLVIGYDPPSAPHPNDLSGPSVPGMGGDGEEDEGDEDRLDNAVRGPGPKGPVGTVSEAQLARRLTKVKNSRRMLSNKPQDFQIRVRVIEGRQLSGNNIRPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVNMTPSELMDEIISIRVYNSHSLRADCLMGEFKIDVGFVYDEPGHAVMRKWLLLNDPEDTSSGSKGYMKVSMFVLGTGDEPPPERRDRDNDSDDVESNLLLPAGIALRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGGNADKKNLVDPFVEVSFAGKKVCTNIIEKNANPEWNQVVNLQIKFPSVCEKIKLTIYDWDRLTKNDVVGTTYLHLSKIAASGGEVEDFSSSGTGAASYTVNTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDELNTGKGEGVAYRGRILVELATFLEKTPPDKKLEPISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDVGEAIQFEVSIGNYGNKFDTTCKPLASTTQYSRAVFDGNYYYYLPWAHTKPVVTLTSYWEDISHRLDAVNTLLAMAERLQTNIEALKSGIQGKIPANQLAELWLKLIDEVIEDTRYTLPLTEGKANVTVLDTQIRKLRSRSLSQIHEAAVRMRSEATDVKSTLAEIEDWLDKLMQLTEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTSGENASGKYCGKTQTIFLKYPQEKNNGPKVPVELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKREFFLPPKGWEWEGEWIVDPERSLLTEADAGHTEFTDEVYQNESRYPGGDWKPAEDTYTDANGDKAASPSELTCPPGWEWEDDAWSYDINRAVDEKGWEYGITIPPDHKPKSWVAAEKMYHTHRRRRLVRKRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGAAAIFKLEGALGADTTEDGDEKSLEKQKHSATTVFGANTPIVSCNFDRVYIYHLRCYVYQARNLLALDKDSFSDPYAHICFLHRSKTTEIIHSTLNPTWDQTIIFDEVEIYGEPQTVLQNPPKVIMELFDNDQVGKDEFLGRSIFSPVVKLNSEMDITPKLLWHPVMNGDKACGDVLVTAELILRGKDGSNLPILPPQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNFQMASITSPSLVVECGGERVESVVIKNLKKTPNFPSSVLFMKVFLPKEELYMPPLVIKVIDHRQFGRKPVVGQCTIERLDRFRCDPYAGKEDIVPQLKASLLSAPPCRDIVIEMEDTKPLLASKLTEKEEEIVDWWSKFYASSGEHEKCGQYIQKGYSKLKIYNCELENVAEFEGLTDFSDTFKLYRGKSDENEDPSVVGEFKGSFRIYPLPDDPSVPAPPRQFRELPDSVPQECTVRIYIVRGLELQPQDNNGLCDPYIKITLGKKVIEDRDHYIPNTLNPVFGRMYELSCYLPQEKDLKISVYDYDTFTRDEKVGETIIDLENRFLSRFGSHCGIPEEYCVSGVNTWRDQLRPTQLLQNVARFKGFPQPILSEDGSRIRYGGRDYSLDEFEANKILHQHLGAPEERLALHILRTQGLVPEHVETRTLHSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKKYYLRVIIWNTKDVILDEKSITGEEMSDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFDYLPAEQLCIVAKKEHFWSIDQTEFRIPPRLIIQIWDNDKFSLDDYLGFLELDLRHTIIPAKSPEKCRLDMIPDLKAMNPLKAKTASLFEQKSMKGWWPCYAEKDGARVMAGKVEMTLEILNEKEADERPAGKGRDEPNMNPKLDLPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLFLLILLLFVAVLLYSLPNYLSMKIVKPNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
91
Sheep
91
Zebrafish
82
Rabbit
82
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZM1 As Substrate

Site PTM Type Enzyme
K89 Ubiquitination
S98 Phosphorylation
Y101 Phosphorylation
K102 Ubiquitination
S105 Phosphorylation
K167 Ubiquitination
T172 Phosphorylation
S174 Phosphorylation
T183 Phosphorylation
S193 Phosphorylation
S212 Phosphorylation
Y288 Phosphorylation
K298 Ubiquitination
T308 Phosphorylation
T325 Phosphorylation
S340 Phosphorylation
K382 Ubiquitination
K391 Ubiquitination
K392 Ubiquitination
K406 Acetylation
K406 Ubiquitination
K407 Acetylation
K407 Ubiquitination
K439 Ubiquitination
K450 Ubiquitination
T456 Phosphorylation
T457 Phosphorylation
Y458 Phosphorylation
S462 Phosphorylation
Y513 Phosphorylation
T514 Phosphorylation
Y520 Phosphorylation
K527 Ubiquitination
K553 Ubiquitination
K567 Ubiquitination
Y619 Phosphorylation
S620 Phosphorylation
T637 Phosphorylation
T642 Phosphorylation
K712 Ubiquitination
S727 Phosphorylation
S729 Phosphorylation
S731 Phosphorylation
Y798 Phosphorylation
K808 Ubiquitination
K812 Ubiquitination
K847 Acetylation
T876 Phosphorylation
K884 Acetylation
K884 Ubiquitination
K891 Ubiquitination
T928 Phosphorylation
T931 Phosphorylation
Y935 Phosphorylation
K947 Ubiquitination
Y953 Phosphorylation
K991 Ubiquitination
Y1015 Phosphorylation
S1036 Phosphorylation
S1037 Phosphorylation
T1038 Phosphorylation
K1064 Methylation
T1070 Phosphorylation
T1102 Phosphorylation
K1109 Ubiquitination
S1110 Phosphorylation
T1126 Phosphorylation
Y1137 Phosphorylation
Y1163 Phosphorylation
T1174 Phosphorylation
T1181 Phosphorylation
K1223 Ubiquitination
S1233 Phosphorylation
K1237 Ubiquitination
S1240 Phosphorylation
K1317 Acetylation
K1344 Ubiquitination
K1348 Ubiquitination
K1365 Acetylation
K1365 Ubiquitination
Y1369 Phosphorylation
K1386 Ubiquitination
T1393 Phosphorylation
K1408 Ubiquitination
K1416 Methylation
K1416 Ubiquitination
S1418 Phosphorylation
K1436 Ubiquitination
S1441 Phosphorylation
K1442 Ubiquitination
T1444 Phosphorylation
K1446 Ubiquitination
S1460 Phosphorylation
S1461 Phosphorylation
K1466 Ubiquitination
K1477 Acetylation
K1507 Ubiquitination
S1508 Phosphorylation
S1515 Phosphorylation
K1521 Ubiquitination
K1585 Ubiquitination
Y1623 Phosphorylation
Y1625 Phosphorylation
K1633 Ubiquitination
S1692 Phosphorylation
Y1705 Phosphorylation
S1706 Phosphorylation
K1714 Ubiquitination
T1781 Phosphorylation
K1805 Ubiquitination
S1806 Phosphorylation
Y1816 Phosphorylation
K1818 Ubiquitination
K1828 Ubiquitination
Y1835 Phosphorylation
K1914 Ubiquitination
S1915 Phosphorylation
K1918 Ubiquitination
K1928 Ubiquitination
K1934 Methylation
K1934 Ubiquitination
K1936 Ubiquitination
K1956 Ubiquitination
K1976 Ubiquitination
K1995 Ubiquitination
T2004 Phosphorylation
S2005 Phosphorylation
Y2051 Phosphorylation
S2053 Phosphorylation

Research Backgrounds

Function:

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).

Subcellular Location:

Cell membrane>Single-pass type II membrane protein. Nucleus membrane>Single-pass type II membrane protein. Cytoplasmic vesicle membrane>Single-pass type II membrane protein.
Note: Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.

Subunit Structure:

Interacts with DNM2 and KDR. Interacts with EHD1 (By similarity). Interacts with EHD2; the interaction is direct. Interacts with RIPOR2.

Family&Domains:

The C2 domain 1 associates with lipid membranes in a calcium-dependent manner.

Belongs to the ferlin family.

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