NAP1L4 Antibody - #DF13174

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Product: NAP1L4 Antibody
Catalog: DF13174
Description: Rabbit polyclonal antibody to NAP1L4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 52 kDa; 43kD(Calculated).
Uniprot: Q99733
RRID: AB_2846134

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
NAP1L4 Antibody detects endogenous levels of total NAP1L4.
RRID:
AB_2846134
Cite Format: Affinity Biosciences Cat# DF13174, RRID:AB_2846134.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NAP2; Nucleosome assembly protein 1-like 4; Nucleosome assembly protein 2;

Immunogens

Immunogen:
Uniprot:

Q99733(NP1L4_HUMAN) >>Visit HPA database.

Gene(ID):
NAP1L4(4676)
Expression:
Q99733 NP1L4_HUMAN:

Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.

Sequence:
MADHSFSDGVPSDSVEAAKNASNTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLPKAVKRRINALKQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAESEWHSENEEEEKLAGDMKSKVVVTEKAAATAEEPDPKGIPEFWFTIFRNVDMLSELVQEYDEPILKHLQDIKVKFSDPGQPMSFVLEFHFEPNDYFTNSVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINPKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99733 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S7 Phosphorylation
S12 Phosphorylation
S14 Phosphorylation
K19 Ubiquitination
T24 Phosphorylation
K26 Ubiquitination
T28 Phosphorylation
T51 Phosphorylation
S53 Phosphorylation
S54 Phosphorylation
Y55 Phosphorylation
T58 Phosphorylation
K61 Ubiquitination
K71 Ubiquitination
Y85 Phosphorylation
K94 Ubiquitination
K105 Ubiquitination
T111 Phosphorylation
T117 Phosphorylation
S121 Phosphorylation
S125 Phosphorylation
S139 Phosphorylation
K140 Ubiquitination
K146 Ubiquitination
T150 Phosphorylation
K192 Ubiquitination
Y215 Phosphorylation
K255 Acetylation
K255 Ubiquitination
K256 Acetylation
K263 Ubiquitination
K282 Ubiquitination
S299 Phosphorylation
S304 Phosphorylation
S309 Phosphorylation

Research Backgrounds

Function:

Acts as histone chaperone in nucleosome assembly.

PTMs:

Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.

Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.
Note: Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.

Subunit Structure:

Interacts with core (H2A, H2B, H3, H4) and linker (H1) histones.

Family&Domains:

Belongs to the nucleosome assembly protein (NAP) family.

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