NCOA5 Antibody - #DF13175

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Product: NCOA5 Antibody
Catalog: DF13175
Description: Rabbit polyclonal antibody to NCOA5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 66 kDa; 66kD(Calculated).
Uniprot: Q9HCD5
RRID: AB_2846135

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NCOA5 Antibody detects endogenous levels of total NCOA5.
RRID:
AB_2846135
Cite Format: Affinity Biosciences Cat# DF13175, RRID:AB_2846135.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA465L10.6; CIA; Coactivator independent of AF 2; KIAA1637; MGC28864; NCoA 5; NCOA5; Nuclear receptor coactivator 5; OTTMUSP00000001057; RP23-61O3.13;

Immunogens

Immunogen:
Uniprot:

Q9HCD5(NCOA5_HUMAN) >>Visit HPA database.

Gene(ID):
NCOA5(57727)
Expression:
Q9HCD5 NCOA5_HUMAN:

Widely expressed.

Sequence:
MNTAPSRPSPTRRDPYGFGDSRDSRRDRSPIRGSPRREPRDGRNGRDARDSRDIRDPRDLRDHRHSRDLRDHRDSRSVRDVRDVRDLRDFRDLRDSRDFRDQRDPMYDRYRDMRDSRDPMYRREGSYDRYLRMDDYCRRKDDSYFDRYRDSFDGRGPPGPESQSRAKERLKREERRREELYRQYFEEIQRRFDAERPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQAAKMADEAILQERERGGPEEGVRGGHPPAIQSLINLLADNRYLTAEETDKIINYLRERKERLMRSSTDSLPGPISRQPLGATSGASLKTQPSSQPLQSGQVLPSATPTPSAPPTSQQELQAKILSLFNSGTVTANSSSASPSVAAGNTPNQNFSTAANSQPQQRSQASGNQPPSILGQGGSAQNMGPRPGAPSQGLFGQPSSRLAPASNMTSQRPVSSTGINFDNPSVQKALDTLIQSGPALSHLVSQTTAQMGQPQAPMGSYQRHY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Horse
92
Bovine
83
Sheep
83
Chicken
83
Xenopus
82
Pig
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCD5 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
S6 Phosphorylation
S9 Phosphorylation
T11 Phosphorylation
R13 Methylation
S21 Phosphorylation
S24 Phosphorylation
S29 Phosphorylation
S34 Phosphorylation
S51 Phosphorylation
S66 Phosphorylation
S75 Phosphorylation
S96 Phosphorylation
Y107 Phosphorylation
S116 Phosphorylation
S126 Phosphorylation
Y127 Phosphorylation
Y136 Phosphorylation
S143 Phosphorylation
Y144 Phosphorylation
S151 Phosphorylation
S162 Phosphorylation
S164 Phosphorylation
Y184 Phosphorylation
S215 Phosphorylation
T267 Phosphorylation
T274 Phosphorylation
K362 Ubiquitination
S377 Phosphorylation
S378 Phosphorylation
T379 Phosphorylation
S381 Phosphorylation
S387 Phosphorylation
T394 Phosphorylation
S395 Phosphorylation
S398 Phosphorylation
S404 Phosphorylation
T418 Phosphorylation
T420 Phosphorylation
S477 Phosphorylation
R500 Methylation
S505 Phosphorylation
R515 Methylation
S529 Phosphorylation
T546 Phosphorylation
T562 Phosphorylation

Research Backgrounds

Function:

Nuclear receptor coregulator that can have both coactivator and corepressor functions. Interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. Involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC in response to 17-beta-estradiol (E2).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Binds HTATIP2/TIP30. Interacts with YLPM1. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, RBMX and PPP1CA.

Family&Domains:

Contains one Leu-Xaa-Xaa-Leu-Leu (LxxLL) motif that is essential for the association with nuclear receptors.

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