NECAB2 Antibody - #DF13179

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Product: NECAB2 Antibody
Catalog: DF13179
Description: Rabbit polyclonal antibody to NECAB2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 42 kDa; 43kD(Calculated).
Uniprot: Q7Z6G3
RRID: AB_2846139

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NECAB2 Antibody detects endogenous levels of total NECAB2.
RRID:
AB_2846139
Cite Format: Affinity Biosciences Cat# DF13179, RRID:AB_2846139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EF hand calcium binding protein 2; EF-hand calcium-binding protein 2; N-terminal EF-hand calcium-binding protein 2; NECA2_HUMAN; Necab2; Neuronal calcium binding protein 2; Neuronal calcium-binding protein 2; Stip 2; Stip-2; Stip2; Synaptotagmin interacting protein 2; Synaptotagmin-interacting protein 2;

Immunogens

Immunogen:
Uniprot:

Q7Z6G3(NECA2_HUMAN) >>Visit HPA database.

Gene(ID):
NECAB2(54550)
Expression:
Q7Z6G3 NECA2_HUMAN:

Expressed in brain. Expressed in the spinal dorsal horn with especially strong expression in lamina IIi; found in excitory synaptic boutons and in ependymal cells (at protein level).

Sequence:
MCERAARLCRAGAHRLLREPPQQGRALGGLLRWVGARMGEPRESLAPAAPADPGPASPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDLFHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Research Backgrounds

Function:

May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.

Subcellular Location:

Cytoplasm. Cell projection>Dendrite. Cell projection>Axon. Cell membrane.
Note: Colocalizes with ADORA2A and/or mGluR5/GRM5 at the plasma membrane (PubMed:17689978, PubMed:19694902). Found in neuronal somata (PubMed:26843217). Detected in the cytoplasm of striatal neurons, at postsynaptic sites, filling dendritic shafts and spines, and at presynaptic sites, filling axon terminals (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain. Expressed in the spinal dorsal horn with especially strong expression in lamina IIi; found in excitory synaptic boutons and in ependymal cells (at protein level).

Subunit Structure:

Interacts (calcium-dependent) with ADORA2A and GRM5.

Restrictive clause

 

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