NECAP1 Antibody - #DF13180
| Product: | NECAP1 Antibody |
| Catalog: | DF13180 |
| Description: | Rabbit polyclonal antibody to NECAP1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 40 kDa; 30kD(Calculated). |
| Uniprot: | Q8NC96 |
| RRID: | AB_2846140 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13180, RRID:AB_2846140.
Immunogens
- Q8NC96 NECP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKTAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQESEISKESQEMDARPKLDLGFKEGQTIKLCIGNITNKKGGASKPRTARGGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGSDADILLDLDSPAPVTTPAPTPVSVSNDLWGDFSTASSSVPNQAPQPSNWVQF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NC96 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R46 | Methylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K154 | Methylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| R180 | Methylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in endocytosis.
Cytoplasmic vesicle>Clathrin-coated vesicle membrane. Cell membrane.
Note: Colocalizes with AP-2 at the plasma membrane.
Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).
The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2 (By similarity).
Belongs to the NECAP family.
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