NELF-A Antibody - #DF13181
| Product: | NELF-A Antibody |
| Catalog: | DF13181 |
| Description: | Rabbit polyclonal antibody to NELF-A |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 57 kDa; 57kD(Calculated). |
| Uniprot: | Q9H3P2 |
| RRID: | AB_2846141 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13181, RRID:AB_2846141.
Fold/Unfold
FLJ10442; FLJ25112; Negative elongation factor A; Negative elongation factor polypeptide A; NELF A; NELF-A; NELFA; NELFA_HUMAN; P/OKcl.15; P/OKcl15; WHSC 2; Whsc2; Wolf Hirschhorn syndrome candidate 2; Wolf Hirschhorn syndrome candidate 2 protein; Wolf-Hirschhorn syndrome candidate 2 protein;
Immunogens
Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
- Q9H3P2 NELFA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H3P2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| C131 | S-Nitrosylation | Uniprot | |
| C141 | S-Nitrosylation | Uniprot | |
| T157 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K161 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K190 | Methylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K200 | Methylation | Uniprot | |
| R202 | Methylation | Uniprot | |
| T212 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| R238 | Methylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K288 | Sumoylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| T297 | Phosphorylation | Uniprot | |
| Y300 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| T365 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K446 | Methylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| Y521 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot |
Research Backgrounds
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.
Nucleus.
Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; NELFA and NELFCD form a stable subcomplex that binds to the N-terminus of NELFB. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure-dependent manner. Interacts with the RNA polymerase II complex when it is not phosphorylated by P-TEFb.
The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.
Belongs to the NELF-A family.
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