NIP30 Antibody | Affinity Biosciences

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NIP30 Antibody - Western blot analysis of extracts from HeLa cells, using NIP30 Antibody.

Product:	NIP30 Antibody
Catalog:	DF13184
Description:	Rabbit polyclonal antibody to NIP30
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	29 kDa; 29kD(Calculated).
Uniprot:	Q9GZU8
RRID:	AB_2846144

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(92%), Dog(91%)

Clonality:

Polyclonal

Specificity:

NIP30 Antibody detects endogenous levels of total NIP30.

RRID:

AB_2846144
Cite Format: Affinity Biosciences Cat# DF13184, RRID:AB_2846144.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C16orf94; CDA018; CDA10; F192A_HUMAN; Fam192a; LOC80011; NEFA interacting nuclear protein; NEFA-interacting nuclear protein NIP30; NIP 30; NIP30; Protein FAM192A;

Immunogens

Immunogen:

Uniprot:

Q9GZU8(PIP30_HUMAN) >>Visit HPA database.

Gene(ID):

FAM192A(80011)

Sequence:

Q9GZU8 PIP30_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGGDDGNLIIKKRFVSEAELDERRKRRQEEWEKVRKPEDPEECPEEVYDPRSLYERLQEQKDRKQQEYEEQFKFKNMVRGLDEDETNFLDEVSRQQELIEKQRREEELKELKEYRNNLKKVGISQENKKEVEKKLTVKPIETKNKFSQAKLLAGAVKHKSSESGNSVKRLKPDPEPDDKNQEPSSCKSLGNTSLSGPSIHCPSAAVCIGILPGLGAYSGSSDSESSSDSEGTINATGKIVSSIFRTNTFLEAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZU8 As Substrate

Q9GZU8

Site	PTM Type	Source
M1	Acetylation	Uniprot
S17	Phosphorylation	Uniprot
Y49	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
K65	Ubiquitination	Uniprot
Y69	Phosphorylation	Uniprot
K74	Ubiquitination	Uniprot
K102	Ubiquitination	Uniprot
K135	Ubiquitination	Uniprot
T137	Phosphorylation	Uniprot
K139	Acetylation	Uniprot
K139	Ubiquitination	Uniprot
T143	Phosphorylation	Uniprot
K151	Acetylation	Uniprot
K151	Ubiquitination	Uniprot
K158	Ubiquitination	Uniprot
K160	Acetylation	Uniprot
K169	Acetylation	Uniprot
K172	Acetylation	Uniprot
K180	Acetylation	Uniprot
C187	S-Nitrosylation	Uniprot
K188	Acetylation	Uniprot
S222	Phosphorylation	Uniprot
S228	Phosphorylation	Uniprot
S242	Phosphorylation	Uniprot
T249	Phosphorylation	Uniprot

Research Backgrounds

Q9GZU8_PIP30_HUMAN

Function:

Promotes the association of the proteasome activator complex subunit PSME3 with the 20S proteasome and regulates its activity. Inhibits PSME3-mediated degradation of some proteasome substrates, probably by affecting their diffusion rate into the catalytic chamber of the proteasome. Also inhibits the interaction of PSME3 with COIL, inhibits accumulation of PSME3 in Cajal bodies and positively regulates the number of Cajal bodies in the nucleus.

PTMs:

Phosphorylation by CK2 stabilizes the interaction with PSME3.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts (via C-terminus) with both free and 20S proteasome-bound forms of the proteasome activator complex subunit PSME3; the interaction is direct.

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NIP30 Antibody - #DF13184