NOMO2 Antibody - #DF13191
(2)
| Product: | NOMO2 Antibody |
| Catalog: | DF13191 |
| Description: | Rabbit polyclonal antibody to NOMO2 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 130 kDa; 139kD(Calculated). |
| Uniprot: | Q5JPE7 |
| RRID: | AB_2846151 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
NOMO2 Antibody detects endogenous levels of total NOMO2.
RRID:
AB_2846151
Cite Format: Affinity Biosciences Cat# DF13191, RRID:AB_2846151.
Cite Format: Affinity Biosciences Cat# DF13191, RRID:AB_2846151.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q5JPE7 NOMO2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLVGQGAGLLGPAVVTAAVVLLLSGVGPAHGSEDIVVGCGGFVKSDVEINYSLIEIKLYTKHGTLKYQTDCAPNNGYFMIPLYDKGDFILKIEPPLGWSFEPTTVELHVDGVSDICTKGGDINFVFTGFSVNGKVLSKGQPLGPAGVQVSLRNTGTEAKIQSTVTQPGGKFAFFKVLPGDYEILATHPTWALKEASTTVRVTNSNANAASPLIVAGYNVSGSVRSDGEPMKGVKFLLFSSLVTKEDVLGCNVSPVPGFQPQDESLVYLCYTVSREDGSFSFYSLPSGGYTVIPFYRGERITFDVAPSRLDFTVEHDSLKIEPVFHVMGFSVTGRVLNGPEGDGVPEAVVTLNNQIKVKTKADGSFRLENITTGTYTIHAQKEHLYFETVTIKIAPNTPQLADIVATGFSVCGRISIIRFPDTVKQMNKYKVVLSSQDKDKSLVTVETDAHGSFCFKAKPGTYKVQVMVPEAETRAGLTLKPQTFPLTVTDRPVMDVAFVQFLASVSGKVSCLDTCGDLLVTLQSLSRQGEKRSLQLSGKVNAMTFTFDNVLPGKYKISIMHEDWCWKNKSLEVEVLEDDVSAVEFRQTGYMLRCSLSHAITLEFYQDGNGRENVGIYNLSKGVNRFCLSKPGVYKVTPRSCHRFEQAFYTYDTSSPSILTLTAIRHHVLGTITTDKMMDVTVTIKSSIDSEPALVLGPLKSVQELRREQQLAEIEARRQEREKNGNEEGEERMTKPPVQEMVDELQGPFSYDFSYWARSGEKITVTPSSKELLFYPPSMEAVVSGESCPGKLIEIHGKAGLFLEGQIHPELEGVEIVISEKGASSPLITVFTDDKGAYSVGPLHSDLEYTVTSQKEGYVLTAVEGTIGDFKAYALAGVSFEIKAEDDQPLPGVLLSLSGGLFRSNLLTQDNGILTFSNLSPGQYYFKPMMKEFRFEPSSQMIEVQEGQNLKITITGYRTAYSCYGTVSSLNGEPEQGVAMEAVGQNDCSIYGEDTVTDEEGKFRLRGLLPGCVYHVQLKAEGNDHIERALPHHRVIEVGNNDIDDVNIIVFRQINQFDLSGNVITSSEYLPTLWVKLYKSENLDNPIQTVSLGQSLFFHFPPLLRDGENYVVLLDSTLPRSQYDYILPQVSFTAVGYHKHITLIFNPTRKLPEQDIAQGSYIALPLTLLVLLAGYNHDKLIPLLLQLTSRLQGVGALGQAASDNSGPEDAKRQAKKQKTRRTLRLQEEFQLMWCLVPWRGTLGIHLFSSLPFASEILLETTATCIHY
PTMs - Q5JPE7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K61 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K159 | Sumoylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K170 | Acetylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K539 | Acetylation | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K630 | Acetylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| Y649 | Phosphorylation | Uniprot | |
| T650 | Phosphorylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| T660 | Phosphorylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| K791 | Ubiquitination | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| S845 | Phosphorylation | Uniprot | |
| Y849 | Phosphorylation | Uniprot | |
| K855 | Ubiquitination | Uniprot | |
| K931 | Ubiquitination | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| K1079 | Ubiquitination | Uniprot | |
| Y1110 | Phosphorylation | Uniprot | |
| Y1123 | Phosphorylation | Uniprot | |
| Y1125 | Phosphorylation | Uniprot | |
| Y1137 | Phosphorylation | Uniprot | |
| S1202 | Phosphorylation | Uniprot | |
| S1205 | Phosphorylation | Uniprot | |
| K1211 | Acetylation | Uniprot | |
| K1211 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NCLN.
Subcellular Location:
Endoplasmic reticulum membrane>Single-pass membrane protein.
Tissue Specificity:
Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
Subunit Structure:
Forms a complex with NCLN and TMEM147, resulting in a stabilization of the 3 proteins, which are otherwise quickly degraded by the proteasome.
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