nucleobindin1 Antibody - #DF13197
| Product: | nucleobindin1 Antibody |
| Catalog: | DF13197 |
| Description: | Rabbit polyclonal antibody to nucleobindin1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 63 kDa; 54kD(Calculated). |
| Uniprot: | Q02818 |
| RRID: | AB_2846157 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13197, RRID:AB_2846157.
Fold/Unfold
CALNUC; FLJ40471; NUC; NUCB 1; NUCB1; NUCB1_HUMAN; Nucleobindin 1; Nucleobindin-1;
Immunogens
Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
- Q02818 NUCB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02818 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| T39 | O-Glycosylation | Uniprot | |
| T42 | O-Glycosylation | Uniprot | |
| S44 | O-Glycosylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T47 | O-Glycosylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S93 | O-Glycosylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| T148 | O-Glycosylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| T160 | O-Glycosylation | Uniprot | |
| T162 | O-Glycosylation | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| S224 | O-Glycosylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| R310 | Methylation | Uniprot | |
| T313 | O-Glycosylation | Uniprot | |
| S320 | O-Glycosylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T321 | O-Glycosylation | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| T343 | Phosphorylation | Uniprot | |
| S369 | O-Glycosylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| T372 | O-Glycosylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S378 | O-Glycosylation | Uniprot | |
| T426 | O-Glycosylation | Uniprot | |
| S444 | O-Glycosylation | Uniprot |
Research Backgrounds
Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
O-glycosylated.
Golgi apparatus>cis-Golgi network membrane>Peripheral membrane protein>Lumenal side. Cytoplasm. Secreted.
Note: A small fraction of the protein may be cytoplasmic.
Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
Interacts (via GBA motif) with guanine nucleotide-binding protein G(i) alpha subunits GNAI1, GNAI2 and GNAI3 with higher affinity for GNAI1 and GNAI3 than for GNAI2. Preferentially interacts with inactive rather than active GNAI3. Interaction with GNAI3 is inhibited when NUCB1 binds calcium, probably due to a conformational change which renders the GBA motif inaccessible.
The EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
The GBA (G-alpha binding and activating) motif mediates binding to the alpha subunits of guanine nucleotide-binding proteins (G proteins).
Belongs to the nucleobindin family.
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