Product: NUS1 Antibody
Catalog: DF13200
Description: Rabbit polyclonal antibody to NUS1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 31 kDa; 33kD(Calculated).
Uniprot: Q96E22
RRID: AB_2846160

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NUS1 Antibody detects endogenous levels of total NUS1.
RRID:
AB_2846160
Cite Format: Affinity Biosciences Cat# DF13200, RRID:AB_2846160.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MTGLYELVWRVLHALLCLHRTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKPPAVGRNRRHHRHPRGGSCLAAAHHRMRWRADGRSLEKLPVHMGLVITEVEQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGLDCSKYSPEFANSNDKDDQVLNCHLAVKVLSPEDGKADIVRAAQDFCQLVAQKQKRPTDLDVDTLASLLSSNGCPDPDLVLKFGPVDSTLGFLPWHIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Pig
91
Rabbit
91
Chicken
60
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96E22 As Substrate

Site PTM Type Enzyme
S45 Phosphorylation
S76 Phosphorylation
N144 N-Glycosylation
K154 Ubiquitination
K166 Ubiquitination
S168 Phosphorylation
K177 Ubiquitination
K189 Ubiquitination
K197 Ubiquitination
K214 Ubiquitination
N271 N-Glycosylation

Research Backgrounds

Function:

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Colocalizes with Nogo-B during VEGF and wound healing angiogenesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms an active dehydrodolichyl diphosphate synthase complex with DHDDS. Interacts with NPC2.

Family&Domains:

Belongs to the UPP synthase family.

Research Fields

· Metabolism > Metabolism of terpenoids and polyketides > Terpenoid backbone biosynthesis.

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