Product: ODF2L Antibody
Catalog: DF13205
Description: Rabbit polyclonal antibody to ODF2L
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 60 kDa; 74kD(Calculated).
Uniprot: Q9ULJ1
RRID: AB_2846165

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(91%)
Clonality:
Polyclonal
Specificity:
ODF2L Antibody detects endogenous levels of total ODF2L.
RRID:
AB_2846165
Cite Format: Affinity Biosciences Cat# DF13205, RRID:AB_2846165.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dJ977L11.1; KIAA1229; MGC111060; ODF2L; ODF2L_HUMAN; Outer dense fiber of sperm tails 2 like; Outer dense fiber protein 2 like; Outer dense fiber protein 2-like; RP5 977L11.1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9ULJ1 ODF2L_HUMAN:

Mainly expressed in trachea and testis. Not detected in bone marrow, bladder, leukocytes. Only weakly detected in tongue, stomach, brain and ovaries.

Sequence:
MEKAVNDGSHSEELFCHLKTISEKEDLPRCTSESHLSCLKQDILNEKTELEATLKEAELVTHSVELLLPLFKDTIEKINFENANLSALNLKISEQKEILIKELDTFKSVKLALEHLLRKRDYKQTGDNLSSMLLENLTDNESENTNLKKKVFEKEAHIQELSCLFQSEKANTLKANRFSQSVKVVHERLQIQIHKREAENDKLKEYVKSLETKIAKWNLQSRMNKNEAIVMKEASRQKTVALKKASKVYKQRLDHFTGAIEKLTSQIRDQEAKLSETISASNAWKSHYEKIVIEKTELEVQIETMKKQIINLLEDLKKMEDHGKNSCEEILRKVHSIEYENETLNLENTKLKLRFPCRITESKNMNILIVLDMLCYISSEKTTLAALKDEVVSVENELSELQEVEKKQKTLIEMYKTQVQKLQEAAEIVKSRCENLLHKNNQITKTKNKNVEKMRGQMESHLKELERVCDSLTAAERRLHECQESLQCCKGKCADQEHTIRELQGQVDGNHNLLTKLSLEEENCLIQLKCENLQQKLEQMDAENKELEKKLANQEECLKHSNLKFKEKSAEYTALARQLEAALEEGRQKVAEEIEKMSSRESALQIKILDLETELRKKNEEQNQLVCKMNSDPETP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Dog
70
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULJ1 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
K19 Ubiquitination
K24 Ubiquitination
S32 Phosphorylation
S34 Phosphorylation
K77 Ubiquitination
K101 Acetylation
K101 Ubiquitination
K169 Ubiquitination
K213 Ubiquitination
K216 Ubiquitination
K262 Ubiquitination
K273 Ubiquitination
K290 Ubiquitination
K333 Ubiquitination
K350 Ubiquitination
T383 Phosphorylation
K409 Acetylation
K416 Acetylation
K463 Ubiquitination
K516 Ubiquitination
K529 Ubiquitination
K550 Ubiquitination
K568 Ubiquitination
K589 Ubiquitination

Research Backgrounds

Function:

Acts as a suppressor of ciliogenesis, specifically, the initiation of ciliogenesis.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrioles in proliferative cells and basal bodies in ciliated cells (PubMed:17485331). Disappears during ciliogenesis but reappears, albeit at a lower levels once ciliogenesis has completed (PubMed:28775150).

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.
Note: Not present in the centrioles in cycling cells. Disappears during ciliogenesis.

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Disappears during ciliogenesis but reappears, albeit at a lower levels once ciliogenesis has completed.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in trachea and testis. Not detected in bone marrow, bladder, leukocytes. Only weakly detected in tongue, stomach, brain and ovaries.

Family&Domains:

Belongs to the ODF2 family.

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