OFD1 Antibody - #DF13207

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Product: OFD1 Antibody
Catalog: DF13207
Description: Rabbit polyclonal antibody to OFD1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog
Mol.Wt.: 43 kDa; 117kD(Calculated).
Uniprot: O75665
RRID: AB_2846167

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(80%), Dog(83%)
Clonality:
Polyclonal
Specificity:
OFD1 Antibody detects endogenous levels of total OFD1.
RRID:
AB_2846167
Cite Format: Affinity Biosciences Cat# DF13207, RRID:AB_2846167.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

71 7A; 717A; CXorf5; JBTS10; Ofd1; OFD1_HUMAN; oral facial digital syndrome 1; Oral-facial-digital syndrome 1 protein; OTTHUMP00000022941; Protein 71-7A; RGD1562231; SGBS2;

Immunogens

Immunogen:
Uniprot:

O75665(OFD1_HUMAN) >>Visit HPA database.

Gene(ID):
OFD1(8481)
Expression:
O75665 OFD1_HUMAN:

Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb.

Sequence:
MMAQSNMFTVADVLSQDELRKKLYQTFKDRGILDTLKTQLRNQLIHELMHPVLSGELQPRSISVEGSSLLIGASNSLVADHLQRCGYEYSLSVFFPESGLAKEKVFTMQDLLQLIKINPTSSLYKSLVSGSDKENQKGFLMHFLKELAEYHQAKESCNMETQTSSTFNRDSLAEKLQLIDDQFADAYPQRIKFESLEIKLNEYKREIEEQLRAEMCQKLKFFKDTEIAKIKMEAKKKYEKELTMFQNDFEKACQAKSEALVLREKSTLERIHKHQEIETKEIYAQRQLLLKDMDLLRGREAELKQRVEAFELNQKLQEEKHKSITEALRRQEQNIKSFEETYDRKLKNELLKYQLELKDDYIIRTNRLIEDERKNKEKAVHLQEELIAINSKKEELNQSVNRVKELELELESVKAQSLAITKQNHMLNEKVKEMSDYSLLKEEKLELLAQNKLLKQQLEESRNENLRLLNRLAQPAPELAVFQKELRKAEKAIVVEHEEFESCRQALHKQLQDEIEHSAQLKAQILGYKASVKSLTTQVADLKLQLKQTQTALENEVYCNPKQSVIDRSVNGLINGNVVPCNGEISGDFLNNPFKQENVLARMVASRITNYPTAWVEGSSPDSDLEFVANTKARVKELQQEAERLEKAFRSYHRRVIKNSAKSPLAAKSPPSLHLLEAFKNITSSSPERHIFGEDRVVSEQPQVGTLEERNDVVEALTGSAASRLRGGTSSRRLSSTPLPKAKRSLESEMYLEGLGRSHIASPSPCPDRMPLPSPTESRHSLSIPPVSSPPEQKVGLYRRQTELQDKSEFSDVDKLAFKDNEEFESSFESAGNMPRQLEMGGLSPAGDMSHVDAAAAAVPLSYQHPSVDQKQIEEQKEEEKIREQQVKERRQREERRQSNLQEVLERERRELEKLYQERKMIEESLKIKIKKELEMENELEMSNQEIKDKSAHSENPLEKYMKIIQQEQDQESADKSSKKMVQEGSLVDTLQSSDKVESLTGFSHEELDDSW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Dog
83
Horse
80
Pig
70
Sheep
60
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75665 As Substrate

Site PTM Type Enzyme
K22 Ubiquitination
K28 Ubiquitination
K37 Ubiquitination
S68 Phosphorylation
K104 Ubiquitination
K125 Ubiquitination
K133 Ubiquitination
K137 Ubiquitination
K154 Ubiquitination
S171 Phosphorylation
K175 Ubiquitination
K192 Ubiquitination
S195 Phosphorylation
K199 Ubiquitination
K218 Ubiquitination
K223 Ubiquitination
K229 Ubiquitination
K256 Ubiquitination
K273 Ubiquitination
K280 Ubiquitination
K291 Ubiquitination
K304 Ubiquitination
K315 Ubiquitination
K322 Ubiquitination
K336 Ubiquitination
K347 Ubiquitination
K358 Ubiquitination
K378 Ubiquitination
S391 Phosphorylation
K392 Ubiquitination
K393 Ubiquitination
S399 Phosphorylation
K404 Ubiquitination
K414 Ubiquitination
S417 Phosphorylation
K422 Ubiquitination
K441 Sumoylation
K441 Ubiquitination
K444 Ubiquitination
K452 Ubiquitination
K455 Ubiquitination
K484 Ubiquitination
K491 Ubiquitination
K509 Ubiquitination
K522 Ubiquitination
K529 Ubiquitination
K533 Ubiquitination
K547 Ubiquitination
Y558 Phosphorylation
K562 Ubiquitination
S586 Phosphorylation
S620 Phosphorylation
S623 Phosphorylation
K632 Ubiquitination
K636 Ubiquitination
S651 Phosphorylation
Y652 Phosphorylation
S660 Phosphorylation
K662 Ubiquitination
S663 Phosphorylation
K668 Ubiquitination
S669 Phosphorylation
S672 Phosphorylation
K680 Ubiquitination
S686 Phosphorylation
T718 Phosphorylation
S720 Phosphorylation
S735 Phosphorylation
S736 Phosphorylation
T737 Phosphorylation
K741 Ubiquitination
S745 Phosphorylation
S762 Phosphorylation
S764 Phosphorylation
S774 Phosphorylation
T776 Phosphorylation
S788 Phosphorylation
S789 Phosphorylation
S811 Phosphorylation
K815 Ubiquitination
S827 Phosphorylation
S830 Phosphorylation
S844 Phosphorylation
K871 Acetylation
K888 Methylation
S899 Phosphorylation
K914 Ubiquitination
S943 Phosphorylation
S951 Phosphorylation
S954 Phosphorylation
K963 Ubiquitination
S973 Phosphorylation
S986 Phosphorylation
S1011 Phosphorylation

Research Backgrounds

Function:

Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Cilium basal body. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.
Note: Localizes to centriole distal ends and to centriolar satellites (PubMed:20230748, PubMed:24121310). Localization to centrioles and pericentriolar satellites may be mediated by KIAA0753/OFIP (PubMed:26643951).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb.

Subunit Structure:

Homooligomer. Interacts with LCA5. Interacts with RUVBL1; the interaction is direct and may mediate interaction with the NuA4 histone acetyltransferase complex. Interacts with SDCCAG8; the interaction is direct. Interacts with MAP1LC3B. Interacts with C2CD3; OFD1 may act as a egative regulator of C2CD3. Forms a complex with KIAA0753/OFIP and FOPNL/FOR20; the interaction with FOPNL is detected only in the presence of KIAA0753. Interacts with PCM1; this interaction may be mediated by KIAA0753/OFIP.

Family&Domains:

Belongs to the OFD1 family.

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