MDC1 Antibody - #DF13250

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Product: MDC1 Antibody
Catalog: DF13250
Description: Rabbit polyclonal antibody to MDC1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 226kDa; 227kD(Calculated).
Uniprot: Q14676
RRID: AB_2846269

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MDC1 Antibody detects endogenous levels of total MDC1.
RRID:
AB_2846269
Cite Format: Affinity Biosciences Cat# DF13250, RRID:AB_2846269.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homologue to Drosophila photoreceptor protein calphotin; MDC 1; Mdc1; MDC1_HUMAN; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; NFBD 1; NFBD1; Nuclear factor with BRCT domains 1; Nuclear Factor with BRCT Domains Protein 1;

Immunogens

Immunogen:
Uniprot:

Q14676(MDC1_HUMAN) >>Visit HPA database.

Gene(ID):
MDC1(9656)
Expression:
Q14676 MDC1_HUMAN:

Highly expressed in testis.

Sequence:
MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14676 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation Q13315 (ATM)
T17 Phosphorylation
S20 Phosphorylation
S21 Phosphorylation
K45 Methylation
K45 Ubiquitination
S94 Phosphorylation
T98 Phosphorylation Q13315 (ATM)
S108 Phosphorylation
S112 Phosphorylation
T146 Phosphorylation
T150 Phosphorylation
T157 Phosphorylation
S168 Phosphorylation
S176 Phosphorylation
S196 Phosphorylation
S202 Phosphorylation
S218 Phosphorylation P68400 (CSNK2A1)
T220 Phosphorylation P68400 (CSNK2A1)
R276 Methylation
S292 Phosphorylation
S299 Phosphorylation P68400 (CSNK2A1)
T301 Phosphorylation P68400 (CSNK2A1)
S307 Phosphorylation
S329 Phosphorylation P68400 (CSNK2A1)
T331 Phosphorylation P68400 (CSNK2A1)
T341 Phosphorylation P24941 (CDK2)
S372 Phosphorylation
S376 Phosphorylation P68400 (CSNK2A1)
T378 Phosphorylation P68400 (CSNK2A1)
S394 Phosphorylation
S397 Phosphorylation
S402 Phosphorylation P68400 (CSNK2A1)
T404 Phosphorylation P68400 (CSNK2A1)
S411 Phosphorylation
S422 Phosphorylation
R429 Methylation
S445 Phosphorylation
T447 Phosphorylation
T448 Phosphorylation
T449 Phosphorylation
S453 Phosphorylation P68400 (CSNK2A1)
T455 Phosphorylation P68400 (CSNK2A1)
K472 Ubiquitination
K476 Ubiquitination
S485 Phosphorylation
S495 Phosphorylation
S498 Phosphorylation
S504 Phosphorylation
S505 Phosphorylation
S513 Phosphorylation Q13535 (ATR)
S516 Phosphorylation
T518 Phosphorylation
T523 Phosphorylation O14757 (CHEK1)
K540 Ubiquitination
S544 Phosphorylation
T548 Phosphorylation
T579 Phosphorylation
S598 Phosphorylation
K616 Sumoylation
K616 Ubiquitination
S641 Phosphorylation
T646 Phosphorylation
T652 Phosphorylation
T654 Phosphorylation
S658 Phosphorylation
T659 Phosphorylation
T699 Phosphorylation
T719 Phosphorylation Q13315 (ATM)
T725 Phosphorylation
T752 Phosphorylation
S760 Phosphorylation
S763 Phosphorylation
T765 Phosphorylation
Y775 Phosphorylation
S780 Phosphorylation
S793 Phosphorylation
T797 Phosphorylation
K812 Acetylation
T847 Phosphorylation
S880 Phosphorylation
S882 Phosphorylation
S955 Phosphorylation
S963 Phosphorylation
S964 Phosphorylation
T966 Phosphorylation
T980 Phosphorylation
S986 Phosphorylation
S988 Phosphorylation
S990 Phosphorylation
S995 Phosphorylation
S998 Phosphorylation
K1016 Acetylation
S1033 Phosphorylation
T1041 Phosphorylation
S1057 Phosphorylation
S1068 Phosphorylation P24941 (CDK2)
K1075 Methylation
T1081 Phosphorylation
S1086 Phosphorylation
S1095 Phosphorylation
K1104 Methylation
T1116 Phosphorylation
T1138 Phosphorylation
K1140 Methylation
T1157 Phosphorylation
S1195 Phosphorylation
T1198 Phosphorylation
S1212 Phosphorylation
S1214 Phosphorylation
T1224 Phosphorylation
T1239 Phosphorylation
S1276 Phosphorylation
T1280 Phosphorylation
T1287 Phosphorylation
S1294 Phosphorylation
T1295 Phosphorylation
S1296 Phosphorylation
T1297 Phosphorylation
T1302 Phosphorylation
S1317 Phosphorylation
S1318 Phosphorylation
T1321 Phosphorylation
T1324 Phosphorylation
T1328 Phosphorylation
T1338 Phosphorylation
T1343 Phosphorylation
K1345 Methylation
T1347 Phosphorylation
S1348 Phosphorylation
T1351 Phosphorylation
T1384 Phosphorylation
S1399 Phosphorylation
S1400 Phosphorylation
K1402 Acetylation
T1403 Phosphorylation
T1406 Phosphorylation
T1410 Phosphorylation
K1413 Sumoylation
T1420 Phosphorylation
T1425 Phosphorylation
T1429 Phosphorylation
S1430 Phosphorylation
T1437 Phosphorylation
S1440 Phosphorylation
S1441 Phosphorylation
T1444 Phosphorylation
T1447 Phosphorylation
S1458 Phosphorylation
T1459 Phosphorylation
T1461 Phosphorylation
T1466 Phosphorylation
S1471 Phosphorylation
S1481 Phosphorylation
S1482 Phosphorylation
T1485 Phosphorylation
T1488 Phosphorylation
S1522 Phosphorylation
S1523 Phosphorylation
T1526 Phosphorylation
T1529 Phosphorylation
T1541 Phosphorylation
T1543 Phosphorylation
T1548 Phosphorylation
T1560 Phosphorylation
S1563 Phosphorylation
S1564 Phosphorylation
T1567 Phosphorylation
S1570 Phosphorylation
S1581 Phosphorylation
T1582 Phosphorylation
T1589 Phosphorylation
T1601 Phosphorylation
S1604 Phosphorylation
S1605 Phosphorylation
T1608 Phosphorylation
T1622 Phosphorylation
T1623 Phosphorylation
S1624 Phosphorylation
T1625 Phosphorylation
T1630 Phosphorylation P24941 (CDK2)
K1648 Acetylation
T1649 Phosphorylation
S1658 Phosphorylation
T1664 Phosphorylation
T1666 Phosphorylation
S1669 Phosphorylation
T1671 Phosphorylation
S1681 Phosphorylation
T1696 Phosphorylation
T1697 Phosphorylation
S1702 Phosphorylation
T1705 Phosphorylation
S1711 Phosphorylation
T1716 Phosphorylation
S1732 Phosphorylation
K1740 Acetylation
K1740 Methylation
S1743 Phosphorylation
S1766 Phosphorylation
S1775 Phosphorylation
T1781 Phosphorylation
S1786 Phosphorylation
S1797 Phosphorylation
T1800 Phosphorylation
S1810 Phosphorylation
K1812 Acetylation
S1814 Phosphorylation
T1817 Phosphorylation
S1820 Phosphorylation
K1840 Sumoylation
K1852 Acetylation
T1858 Phosphorylation
K1885 Ubiquitination
R1904 Methylation
S1915 Phosphorylation
R1943 Methylation
T1971 Phosphorylation
K1977 Ubiquitination
K2075 Ubiquitination

Research Backgrounds

Function:

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

PTMs:

Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain.

Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation.

Ubiquitinated by RNF4, leading to proteasomal degradation; undergoes 'Lys-48'-linked polyubiquitination.

Subcellular Location:

Nucleus. Chromosome.
Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis.

Subunit Structure:

Homodimer. Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with H2AX, which requires phosphorylation of H2AX on 'Ser-139'. Interacts with the MRN complex, composed of MRE11, RAD50, and NBN. Interacts with CHEK2, which requires ATM-mediated phosphorylation of 'Thr-68' within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of XRCC6/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8 (via FHA domain). Interacts with CEP164. When phosphorylated, interacts with APTX (via FHA-like domain).

Family&Domains:

Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AX.

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