CENPB Antibody - #DF13261
| Product: | CENPB Antibody |
| Catalog: | DF13261 |
| Description: | Rabbit polyclonal antibody to CENPB |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine |
| Mol.Wt.: | 65kDa; 65kD(Calculated). |
| Uniprot: | P07199 |
| RRID: | AB_2846280 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13261, RRID:AB_2846280.
Fold/Unfold
CENP B; CENP-B; CENPB; CENPB_HUMAN; centromere autoantigen B; Centromere protein B; Centromere protein B, 80kDa; Major centromere autoantigen B;
Immunogens
- P07199 CENPB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07199 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Methylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| K70 | Sumoylation | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot |
Research Backgrounds
Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).
Poly-ADP-ribosylated by PARP1.
N-terminally methylated by METTL11A/NTM1. Alpha-N-methylation is stimulated in response to extracellular stimuli, including increased cell density and heat shock, and seems to facilitate binding to CENP-B boxes. Chromatin-bound CENP-B is primarily trimethylated.
Nucleus. Chromosome>Centromere.
Antiparallel homodimer. Interacts with CENPT. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1.
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