LAP2 alpha Antibody | Affinity Biosciences

Product:	LAP2 alpha Antibody
Catalog:	DF13264
Description:	Rabbit polyclonal antibody to LAP2 alpha
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	75kDa; 75kD(Calculated).
Uniprot:	P42166
RRID:	AB_2846283

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

LAP2 alpha Antibody detects endogenous levels of total LAP2 alpha.

RRID:

AB_2846283
Cite Format: Affinity Biosciences Cat# DF13264, RRID:AB_2846283.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Isoforms beta/gamma; Lamina associated polypeptide 2; LAP2; Thymopoietin; Thymopoietin isoform alpha; TMPO; TP alpha;

Immunogens

Immunogen:

Uniprot:

P42166(LAP2A_HUMAN) >>Visit HPA database.

Gene(ID):

TMPO(7112)

Expression:

P42166 LAP2A_HUMAN:

Expressed in many tissues. Most abundant in adult thymus and fetal liver.

Sequence:

P42166 LAP2A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEGKKKEHKKVKSTRDIVPFSELGTTPSGGGFFQGISFPEISTRPPLGSTELQAAKKVHTSKGDLPREPLVATNLPGRGQLQKLASERNLFISCKSSHDRCLEKSSSSSSQPEHSAMLVSTAASPSLIKETTTGYYKDIVENICGREKSGIQPLCPERSHISDQSPLSSKRKALEESESSQLISPPLAQAIRDYVNSLLVQGGVGSLPGTSNSMPPLDVENIQKRIDQSKFQETEFLSPPRKVPRLSEKSVEERDSGSFVAFQNIPGSELMSSFAKTVVSHSLTTLGLEVAKQSQHDKIDASELSFPFHESILKVIEEEWQQVDRQLPSLACKYPVSSREATQILSVPKVDDEILGFISEATPLGGIQAASTESCNQQLDLALCRAYEAAASALQIATHTAFVAKAMQADISQAAQILSSDPSRTHQALGILSKTYDAASYICEAAFDEVKMAAHTMGNATVGRRYLWLKDCKINLASKNKLASTPFKGGTLFGGEVCKVIKKRGNKH

PTMs - P42166 As Substrate

P42166

Site	PTM Type	Enzyme	Source
T57	Phosphorylation		Uniprot
S59	Phosphorylation		Uniprot
S66	Phosphorylation		Uniprot
S67	Phosphorylation		Uniprot
T74	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
T154	Phosphorylation		Uniprot
S156	Phosphorylation		Uniprot
T160	Phosphorylation		Uniprot
T164	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S180	Phosphorylation		Uniprot
R182	Methylation		Uniprot
Y183	Phosphorylation		Uniprot
S184	Phosphorylation		Uniprot
T211	Phosphorylation		Uniprot
T212	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
K248	Ubiquitination		Uniprot
T259	Phosphorylation		Uniprot
R264	Methylation		Uniprot
K269	Sumoylation		Uniprot
K269	Ubiquitination		Uniprot
S272	Phosphorylation		Uniprot
S279	Phosphorylation		Uniprot
K281	Methylation		Uniprot
K281	Sumoylation		Uniprot
K281	Ubiquitination		Uniprot
K290	Ubiquitination		Uniprot
S296	Phosphorylation		Uniprot
S306	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
S310	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
K315	Ubiquitination		Uniprot
K323	Ubiquitination		Uniprot
K334	Ubiquitination		Uniprot
S335	Phosphorylation		Uniprot
C341	S-Nitrosylation		Uniprot
S348	Phosphorylation		Uniprot
S351	Phosphorylation		Uniprot
S354	Phosphorylation		Uniprot
S355	Phosphorylation		Uniprot
K356	Acetylation		Uniprot
K356	Sumoylation		Uniprot
K356	Ubiquitination		Uniprot
K358	Ubiquitination		Uniprot
S365	Phosphorylation		Uniprot
S366	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
K410	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
S424	Phosphorylation	P06493 (CDK1)	Uniprot
K428	Ubiquitination		Uniprot
S433	Phosphorylation		Uniprot
K435	Acetylation		Uniprot
K435	Ubiquitination		Uniprot
S436	Phosphorylation		Uniprot
S442	Phosphorylation		Uniprot
S444	Phosphorylation		Uniprot
S459	Phosphorylation		Uniprot
T471	Phosphorylation		Uniprot
K478	Ubiquitination		Uniprot
K484	Ubiquitination		Uniprot
S497	Phosphorylation		Uniprot
K500	Ubiquitination		Uniprot
R511	Methylation		Uniprot
K519	Ubiquitination		Uniprot
K620	Ubiquitination		Uniprot
C629	S-Nitrosylation		Uniprot
T642	Phosphorylation		Uniprot
T647	Phosphorylation		Uniprot
K656	Acetylation		Uniprot
K656	Ubiquitination		Uniprot
K659	Ubiquitination		Uniprot
K665	Ubiquitination		Uniprot
K667	Sumoylation		Uniprot
K667	Ubiquitination		Uniprot
S670	Phosphorylation		Uniprot
T671	Phosphorylation		Uniprot
K674	Methylation		Uniprot
K674	Sumoylation		Uniprot
K674	Ubiquitination		Uniprot
T677	Phosphorylation		Uniprot
K685	Ubiquitination		Uniprot

Research Backgrounds

P42166_LAP2A_HUMAN

Function:

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1.

TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

PTMs:

Phosphorylated in a mitose-specific manner.

Subcellular Location:

Nucleus. Chromosome.
Note: Expressed diffusely throughout the nucleus.

Tissue Specificity:

Expressed in many tissues. Most abundant in adult thymus and fetal liver.

Subunit Structure:

Interacts with LMNA, BANF1 and RB1 and with chromosomes. Associates directly or indirectly with lamins at specific cell-cycle stages. Interacts with CMTM6.

Family&Domains:

The N-terminal part contains two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1.

The C-terminal domain forms a four-stranded coiled coil.

Belongs to the LEM family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

LAP2 alpha Antibody - #DF13264