Casein Antibody - #DF13271

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Product: Casein Antibody
Catalog: DF13271
Description: Rabbit polyclonal antibody to Casein
Application: WB IHC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 21kDa; 22kD(Calculated).
Uniprot: P47710
RRID: AB_2846290

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Casein Antibody detects endogenous levels of total Casein.
RRID:
AB_2846290
Cite Format: Affinity Biosciences Cat# DF13271, RRID:AB_2846290.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha casein; alpha-S1-casein; CASA; CASA1_HUMAN; Casein alpha s1; casein, alpha; casein, alpha-S1, included; Casoxin-D; CSN1; CSN1S1; Csna; MGC149368;

Immunogens

Immunogen:

A synthesized peptide derived from human Casein, corresponding to a region within N-terminal amino acids.

Uniprot:

P47710(CASA1_HUMAN) >>Visit HPA database.

Gene(ID):
CSN1S1(1446)
Expression:
P47710 CASA1_HUMAN:

Mammary gland specific. Secreted in milk.

Sequence:
MRLLILTCLVAVALARPKLPLRYPERLQNPSESSEPIPLESREEYMNGMNRQRNILREKQTDEIKDTRNESTQNCVVAEPEKMESSISSSSEEMSLSKCAEQFCRLNEYNQLQLQAAHAQEQIRRMNENSHVQVPFQQLNQLAAYPYAVWYYPQIMQYVPFPPFSDISNPTAHENYEKNNVMLQW

Research Backgrounds

Function:

Important role in the capacity of milk to transport calcium phosphate.

Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.

PTMs:

Not glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mammary gland specific. Secreted in milk.

Family&Domains:

Belongs to the alpha-casein family.

References

1). A Comparative Analysis of the Gene Expression Profiles in the Mammary Glands of Lactating and Nonlactating Mares at the Second Month of Gestation. Animals : an open access journal from MDPI, 2024 (PubMed: 39199853) [IF=2.7]

Application: IHC    Species: Mouse    Sample:

Figure 5. (A) Immunohistochemistry of CSN3, CSN1S1, LALBA, and LTFCSN3 proteins in mammary tissue of lactating pregnant mares. (B) Quantitative statistics for (A). (C) qPCR was used to detect expressions of CSN3, CSN1S1, LALBA, and LTF genes in equine mammary tissues. * indicates p < 0.05. Data are presented as mean ± SD (n = 3).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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