TDP1 Antibody - #DF13281
| Product: | TDP1 Antibody |
| Catalog: | DF13281 |
| Description: | Rabbit polyclonal antibody to TDP1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Horse, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 68kDa; 68kD(Calculated). |
| Uniprot: | Q9NUW8 |
| RRID: | AB_2846300 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13281, RRID:AB_2846300.
Fold/Unfold
AI838772; AW493413; FLJ11090; MGC104252; MGC112732; RP24-311F12.2; SCAN1; TDP1; TYDP; TYDP1_HUMAN; Tyr-DNA phosphodiesterase 1; Tyrosyl-DNA phosphodiesterase 1;
Immunogens
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
- Q9NUW8 TYDP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSEAQKAAHKRKISPVKFSNTDSVLPPKRQKSGSQEDLGWCLSSSDDELQPEMPQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKEEEDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSPLYPRIADGTHKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSETNVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPNAESWPVVGQFSSVGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NUW8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K22 | Sumoylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Q13315 (ATM) , P78527 (PRKDC) | Uniprot |
| S90 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S92 | Phosphorylation | Uniprot | |
| K112 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K139 | Sumoylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K367 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Q13315 (ATM) | Uniprot |
Research Backgrounds
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
Nucleus. Cytoplasm.
Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
Monomer.
Belongs to the tyrosyl-DNA phosphodiesterase family.
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