KDM4B / JMJD2B Antibody - #DF13288
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13288, RRID:AB_2846307.
Fold/Unfold
JHDM3B; JmjC domain-containing histone demethylation protein 3B; JMJD2B; Jumonji domain containing 2B; Jumonji domain containing protein 2B; Jumonji domain-containing protein 2B; Kdm4b; KDM4B_HUMAN; KIAA0876; Lysine (K) specific demethylase 4B; Lysine demethylase 4B; Lysine-specific demethylase 4B; TDRD14B; Tudor domain containing 14B;
Immunogens
- O94953 KDM4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTALTSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALLEEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKAKSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEESPLPAPLNVVPPEVPSEELEAKPRPIIPMLYVVPRPGKAAFNQEHVSCQQAFEHFAQKGPTWKEPVSPMELTGPEDGAASSGAGRMETKARAGEGQAPSTFSKLKMEIKKSRRHPLGRPPTRSPLSVVKQEASSDEEASPFSGEEDVSDPDALRPLLSLQWKNRAASFQAERKFNAAAARTEPYCAICTLFYPYCQALQTEKEAPIASLGKGCPATLPSKSRQKTRPLIPEMCFTSGGENTEPLPANSYIGDDGTSPLIACGKCCLQVHASCYGIRPELVNEGWTCSRCAAHAWTAECCLCNLRGGALQMTTDRRWIHVICAIAVPEARFLNVIERHPVDISAIPEQRWKLKCVYCRKRMKKVSGACIQCSYEHCSTSFHVTCAHAAGVLMEPDDWPYVVSITCLKHKSGGHAVQLLRAVSLGQVVITKNRNGLYYRCRVIGAASQTCYEVNFDDGSYSDNLYPESITSRDCVQLGPPSEGELVELRWTDGNLYKAKFISSVTSHIYQVEFEDGSQLTVKRGDIFTLEEELPKRVRSRLSLSTGAPQEPAFSGEEAKAAKRPRVGTPLATEDSGRSQDYVAFVESLLQVQGRPGAPF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94953 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K26 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| Y196 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K384 | Acetylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K409 | Acetylation | Uniprot | |
| K409 | Sumoylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K557 | Ubiquitination | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S579 | Phosphorylation | Uniprot | |
| K602 | Acetylation | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| K723 | Ubiquitination | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| K907 | Ubiquitination | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| K928 | Ubiquitination | Uniprot | |
| K1032 | Ubiquitination | Uniprot | |
| K1056 | Ubiquitination | Uniprot | |
| T1065 | Phosphorylation | Uniprot | |
| T1069 | Phosphorylation | Uniprot | |
| Y1078 | Phosphorylation | Uniprot |
Research Backgrounds
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
Nucleus.
The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails (By similarity).
Belongs to the JHDM3 histone demethylase family.
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